Diabetes Mellitus Type 2 in Obese, Obesity
Conditions
Keywords
Insulin resistance, Exercise, Type 2 diabetes mellitus, Obesity, Epigenetic studies
Brief summary
The investigators are trying to understand the role of DNA (deoxyribonucleic acid) methylation in insulin resistance in skeletal muscle and blood tissues. DNA methylation is a normal chemical process in the body that modifies DNA. By studying this, the investigators hope to better understand the causes of insulin resistance.
Detailed description
Insulin resistance is defined as the decreased ability of insulin to perform its biological function in the muscle, liver and fat. Genetic and environmental factors are known to influence insulin sensitivity. It is not known how this is mediated. This study looks at the role of epigenetics (modifications of proteins associated with DNA and methylation of DNA) in alterations in insulin resistance. We will study lean healthy people, obese non-diabetic people and people with type 2 diabetes to characterize the DNA methylation patterns in muscle in each group. The second aim of the study is to see how a single bout of exercise affects the DNA methylation in the muscle. The third aim looks at the effect of 8 weeks of supervised exercise on the DNA methylation.
Interventions
None listed
Sponsors
Mayo Clinic
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
21 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
Volunteers must be: * 21 - 55 years old * must be non-lactating, non-pregnant * not taking medications known to affect glucose or if taking them, on stable doses. * free of significant heart or lung disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| DNA methylation of genes in insulin resistance | Baseline to visit 33 (approx 2 months) | DNA methylation of genes involved in mitochondrial biogenesis, oxidative phosphorylation, extracellular matrix and cytoskeleton proteins in insulin resistance, with an acute episode of exercise, and with eight weeks of training exercise. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| mRNA expression of genes | Baseline to visit 33 approx 2 months | mRNA expression of genes involved in mitochondrial biogenesis, oxidative phosphorylation, extracellular matrix and cytoskeletal signaling are altered in insulin resistance, with an acute episode of exercise and with 8 weeks of exercise training. |
Countries
United States
Outcome results
None listed