Postprandial IHL and IMCL Dynamics
Conditions
Brief summary
The major research objective is to assess the potential of Proton Magnetic Resonance Spectroscopy to follow Intrahepatic lipid and Intramyocellular lipid dynamics after a single meal. Furthermore we aim to investigate whether the addition of protein to a high fat breakfast results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast alone.
Detailed description
Fat accumulation in non-adipose tissue, such as liver and muscle, is associated with reduced insulin sensitivity and cardiovascular disease. Little is known about the dietary determinants and the time course of this ectopic fat accumulation. Therefore the general aim of this project is to develop and validate methodology to follow postprandial hepatic and muscular lipid retention. Proton Magnetic Resonance Spectroscopy (1H-MRS) is a well-established tool for noninvasive quantification of intrahepatic lipids (IHL) and intramyocellular lipids (IMCL). Previous studies have suggested a relatively rapid regulation of IHL and IMCL levels, indicated by changes in these total lipid pools after high fat diets of 3 or 4 days. Although IHL and IMCL levels seem to change rapidly, data on IHL and IMCL levels after a single meal are lacking.
Interventions
DIETARY_SUPPLEMENTHigh fat breakfast
The HF breakfast will consist of sausage rolls (saucijzenbroodjes), available at the local Albert Heijn (Albert Heijn B.V., Zaandam, The Netherlands).
DIETARY_SUPPLEMENTHF breakfast with protein
For the HFP breakfast, the subjects will consume 'Protifar' (Nutricia, Cuijk, The Netherlands) in addition to the sausage rolls.
Sponsors
Top Institute Food and Nutrition
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 18-40 years * Healthy * Stable dietary habits * No use of medication * Lean subjects: BMI 18-25 kg/m2 * Obese subjects: BMI 30-35 kg/m2
Exclusion criteria
* Any medical condition requiring treatment and/or medication use * Alcohol consumption of more than 20 g per day (± 2 units) * Smoking * Unstable body weight (weight gain or loss \> 3 kg in the past three months) * Participation in another biomedical study within 1 month prior to the screening visit * Contraindications for MRI scan: * Central nervous system aneurysm clips * Implanted neural stimulator * Implanted cardiac pacemaker of defibrillator * Cochlear implant * Iron- containing corpora aliena in the eye or brain * Hearing aids and artificial (heart) valves which is contraindicated for MRS * Claustrophobia
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference between pre- and postprandial IHL and IMCL | 3 and 5 hours after high fat breakfast | The primary research objective is to assess the potential of 1H-MRS to follow IHL and IMCL dynamics after a single meal in lean and obese subjects. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Difference between postprandial IHL and IMCL in high fat and high fat with extra protein breakfast | 3 and 5 hours after high fat breakfast | We aim to investigate whether the addition of protein to a high fat breakfast (HFP) results in a reduced postprandial accumulation of lipids when compared to a high fat breakfast (HF) alone. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Blood plasma glucose levels | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast | — |
| Fat and fat free mass of subjects | One week before the first MR measurements | Body composition of the subjects is measured with underwaterweighing method |
| Blood plasma triglyceride level | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast | — |
| Blood plasma free fatty acid level | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast | — |
| Blood plasma insulin levels | Ten minutes prior to high fat breakfast and 0.5, 1,2,3.5,4.5,5.5 hours after the high fat breakfast | — |
Countries
Netherlands
Outcome results
None listed