The Effects of Amino Acid Supplement During Acute Inflammation.

Conditions

Febrile Illness Acute, Inflammation, Metabolic Diseases

Brief summary

The purpose of the study is to investigate the metabolic effects during acute inflammation with and without the nutritional supplement of amino acids. E. Coli Endotoxin (LPS, lipopolysaccharide) is used to initiate an inflammatory response. The study is an interventional randomized placebo study including 8 healthy male subjects. Each subject participates 3 times (different days) and are given one of following interventions: * Placebo (NaCl) * Endotoxin, US standard reference E.Coli + Placebo (NaCl) * Endotoxin, US standard reference E.Coli + Amino acids (intravenously) It is our hypothesis that the nutritional intervention during acute inflammation plays an important role in lipid and protein metabolism.

Mette Glavind Bülow Pedersen, Esben Stistrup Lauritzen, Mads Svart, Julie Støy, Esben Søndergaard et al. (11 authors)

American Journal of Clinical Nutrition2023-10-1416 citations

10.1016/j.ajcnut.2023.10.007

Regulation of Lipolysis and Adipose Tissue Signaling during Acute Endotoxin-Induced Inflammation: A Human Randomized Crossover Trial

Nikolaj Rittig, Ermina Bach, Henrik Holm Thomsen, Steen B. Pedersen, Thomas S. Nielsen et al. (8 authors)

PLoS ONE2016-09-1464 citations

10.1371/journal.pone.0162167

Amino acid supplementation is anabolic during the acute phase of endotoxin-induced inflammation: A human randomized crossover trial.

Rittig N, Bach E, Thomsen HH, Johannsen M, Jørgensen JO, Richelsen B, Richelsen B, Richelsen B, Jessen N, Møller N.

2015-04-0932 citations

10.1016/j.clnu.2015.03.021

Interventions

BIOLOGICALE. coli endotoxin, US standard

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

TREATMENT

Masking

SINGLE (Subject)

Eligibility

Sex/Gender

MALE

Age

25 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* male gender * 20\<BMI\<30 * Age \> 25 years old * Written consent before starting the study

Exclusion criteria

* Allergy for soya products ore egg * Diabetes * Epilepsy * Infection * Immune defects * Heart disease * Dysregulated hypertension * Participating in other trials using radioactive tracers or x-rays the last year.

Design outcomes

Primary

Measure	Time frame	Description
Protein catabolism during acute illness	After 6 hours intervention	Protein catabolism is measured with amino acid tracer techniques comparing radioactive marked amino acids from blood samples.

Secondary

Measure	Time frame	Description
The local effects on muscle and fat tissue	At 2 hours and 4,5 hours	A muscle + fat biopsy are taken twice during the intervention to focus on the local effects in these tissues.
Other systemic metabolic effects during acute illness	6 hours	Lipid and glucose metabolism are measured with tracer techniques with radioactive marked glucose + lipid.

Countries

Denmark

Outcome results

None listed