Recurrent Pregnancy Losses
Conditions
Keywords
uterine natural killer cells, recurrent miscarriages, alloimmunity
Brief summary
The aim of the study is to evaluate the role of uterine natural killer cell(uNK)in recurrent pregnancy losses and the correlation between the numbers of it and embryo toxicity by measuring the level of the Th2 cytokines in normal reproductive profile and in patients with Recurrent Pregnancy Losses (RPL).
Detailed description
Office hysteroscopy examination of the patients at the luteal phase of the natural cycle (day17 to day 20),with endometrial biopsy using pipple curette from the region of the fundus, the biopsy sent to flow cytometry laboratory in South Egypt Cancer Institute to be examined for: 1.1. Phenotypic analysis of uterine natural killer cells: 1.2. Induction and detection of intracellular cytokines by flow Cytometry:
Interventions
DEVICEoffice hysteroscopy
Endometrial wash and biopsy is taken at the day around implantation window (day 14\_to day 22)of the menstrual cycle from patients of recurrent miscarriages to evaluate the levels of uterine natural killer cells compared to control of females of normal reproductive outcome
Sponsors
Woman's Health University Hospital, Egypt
Study design
Allocation
NON_RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
SCREENING
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
1. Women with unexplained recurrent miscarriages (2 or more consecutive). 2. All patients have normal uterine cavity diagnosed by HSG and /or hysteroscopy, normal semen analysis, normal thrombophilic profiles, no history of malformed 3. normal fetus in the previous miscarriages
Exclusion criteria
1. Age above forty years old . 2. Antiphospholipid syndrome (lupus anticoagulant and/or anticardiolipin antibodies \[IgG or IgM\]); other recognised thrombophilic conditions (testing according to usual clinic practice). 3. Intrauterine abnormalities (as assessed by ultrasound, hysterosonography, hysterosalpingogram, or hysteroscopy). 4. Fibroids distorting uterine cavity . 5. Abnormal parental karyotype . 6. Other identifiable causes of recurrent miscarriages (tests initiated only if clinically indicated) e.g., diabetes, thyroid disease and systemic lupus erythematosus (SLE).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| the role of uterine natural killer cell(uNK)in recurrent pregnancy losses and the correlation between the numbers of it and embryo toxicity by measuring the level of the Th2 cytokines in normal reproductive profile and in patients with RPL. | two years |
Secondary
| Measure | Time frame |
|---|---|
| to set in the horizon the role of immunomodulators as a treatment for RPL and implantation failure | two years |
Countries
Egypt
Outcome results
None listed