Cardiovascular Function, Triglyceride Metabolism
Conditions
Brief summary
Grape seed proanthocyanidins have been reported to possess a hypotriglyceridemic effect after an acute or chronic consumption in rodents. In this study, we aimed to investigate the effects of an acute consumption of proanthocyanidins before a breakfast meal in humans. Moreover, the mechanisms implicated in the reduction of plasma triglycerides levels by grape seed proanthocyanidins in humans should be elucidated as well as its activity on vascular function.
Detailed description
The intervention will consist in the administration of 2 capsules containing the grape seed proanthocyanidins extract or 2 empty capsules (placebo) just before breakfast, which will consist of 100g of white bread, 75g of salami, 50g of fatty cheese, 125g of yogurt (10% fat), 25ml of olive oil and water ad libitum. The nutritional composition of this meal is as follows: 1.200 Kcal of energy content, 15% of energy from proteins, 22% from carbohydrates and 63% from fat (35% saturated fat), and 120mg of cholesterol. Blood samples will be taken at point 0.5,1,2,3 and 6 hours after breakfast. Moreover, urine samples will be collected at time 0 and 6 hours.
Interventions
DIETARY_SUPPLEMENT35 mg/Kg of grape seed proanthocyanidins extract (2 capsules)
OTHER2 empty capsules
Sponsors
University Rovira i Virgili
Hospital Universitari Sant Joan de Reus
Technological Centre of Nutrition and Health, Spain
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Adults between the ages of 20 and 40 * Signing the informed consent
Exclusion criteria
* Body mass index (BMI) lower than 18.5 or greater than 27 Kg/m2 * Smokers * Regular medication * Consumption of medication during the last week * Following a weight-loss diet * Vegetarians * Abnormal glucose levels * Diabetes * Cardiovascular disease * Gastrointestinal disease * Anemia * Nuts allergy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Area under the curve of plasma triglycerides concentration | 0-180 minutes |
Secondary
| Measure | Time frame |
|---|---|
| Endothelial function (assessed by ischemic reactive hyperemia) | 0-0.5-1-2-3-6 hours |
| Blood lipid levels, markers of inflammation and oxidation | 0-0.5-1-2-3-6 hours |
| Plasma levels of procyanidins metabolites | 0-0.5-1-2-3-6 hours |
| Blood pressure | 0-0.5-1-2-3-6 hours |
| Membrane lipid composition of erythrocytes and oxidation markers. | 0-0.5-1-2-3-6 hours |
| Non-targeted metabolomics and 8-epi-prostaglandin-F2α levels (in urine) | 0-6 hours |
| Markers of cholesterol metabolism in PBMCs (mRNA and protein). | 0-0.5-1-2-3-6 hours |
Countries
Spain
Outcome results
None listed