Type 2 Diabetes
Conditions
Brief summary
Inadequate suppression of intramuscular and adipose tissue lipolysis, and consequent excessive delivery of fatty acids to ectopic tissues (e.g. muscle, pancreas and liver) could play an important role in the development and exacerbating of insulin resistance. Therefore, the investigators propose to study the regulation of adipose tissue and skeletal muscle lipolysis, as well as further characterize the intracellular lipolytic pathways within these tissues, in obese normoglycaemic versus long-term diagnosed type 2 diabetic subjects.
Interventions
Sponsors
Netherlands Organisation for Scientific Research
European Foundation for the Study of Diabetes
Maastricht University Medical Center
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
45 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Caucasian * Normal blood pressure (SBP 100-140 mmHg, DBP 60-90 mmHg) * weight stable in last 3 months
Exclusion criteria
* Smokers * people with intensive fitness training (e.g. athletes \> 3 times/week) * History of cardiovascular diseases * Bleeding disorders * Use of medication interfering with the study endpoints/hypotheses (e.g. beta-blockers) * Not to be able to understand the study information * Subjects on a special diet or vegetarian * Blood donation 2 months prior to the study and during the study * Participating in an other study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change from baseline in insulin sensitivity of adipose tissue and skeletal muscle lipolysis | 8, 20 and 40 mU insulin (2 hours) | Changes from baseline in glycerol (lactate, pyruvate and glucose) in tissue microdialysate during a 3 step euglycemic hyperinsulinemic clamp (8, 20 and 40mU of insulin), every step for 2h |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Baseline adipose tissue and skeletal muscle gene expression | baseline | gene expression of genes related to lipid, glucose metabolism, insulin signalling will be measured in baseline adipose tissue and skeletal muscle biopsies |
| Baseline skeletal muscle lipid accumulation | Baseline | Baseline skeletal muscle lipid accumulation is measured (TAG, DAG, Phospholipid and fatty acids) |
Countries
Netherlands
Outcome results
None listed