Breast Cancer
Conditions
Keywords
adipose-derived stem cells, viable ADSCs, apoptosis, necrosis, cryopreservation
Brief summary
The overall purpose of this study is to enhance identification and isolation of ADSCs and adipocytes, to find the optimum culturing conditions to increase growth and volume of grafts, and to study the effects of cryopreservation on ADSCs and adipocytes. Fat injections are used in aesthetic surgery but tend to disassemble over time. If the investigators can enhance growth and volume of fat in culture, the investigators could use fat grafts in reconstruction of cancer and traumatic injury defects with the patients own cultured fat cells and grafts.
Interventions
None listed
Sponsors
University of Nevada, Las Vegas
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* All adults (18-70 yrs) who have scheduled surgical lipectomy of the hips, abdomen, or thighs are prospective subjects for this study.
Exclusion criteria
* Pregnant women will be excluded to protect the unborn child.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Percent of Viable ADSCs | Day of Surgery | Percent of viable ADSCs will be measured on fresh samples and samples refrigerated for different times. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Percent of Apoptotic ADSCs | Day of Surgery | Percent of apoptotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days refrigerated storage. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Percent of Necrotic ADSCs | Day of Surgery | Percent of necrotic ADSCs will be measured on fresh samples and on refrigerated samples at 1, 2, 3, and 4 days of refrigerated storage. |
Countries
United States
Contacts
Primary ContactWilliam A Zamboni, M.D.
Backup ContactShelley J Williams, M.S.
Outcome results
None listed