Female Infertility
Conditions
Keywords
advanced maternal age, infertility, IMSI, ICSI, implantation
Brief summary
The objective of this study was to evaluate the advanced maternal age as a rationale for performing intracytoplasmic morphologically selected sperm injection (IMSI). This prospective randomized study included couples undergoing intracytoplasmic sperm injection (ICSI) as a result of advanced maternal age (≥ 37 years old). Couples were randomly allocated to receive one of two sperm selection procedures (ICSI, n = 33; or IMSI, n = 33). The groups were compared with regard to fertilization rate, percentage of high-quality embryos, implantation, pregnancy and miscarriage rates.
Interventions
ICSI is routinely used to treat couples with infertility. Sperm selection in the ICSI group was analysed under a magnification of 400x using an inverted microscope. ICSI was performed in a micro-injection dish prepared with 4 µL droplets of buffered medium (Global® w/HEPES, LifeGlobal, Connecticut, USA) and covered with paraffin oil on a heated stage at 37.0 ± 0.5°C of an inverted microscope.
IMSI is an established modified ICSI procedure that used an unstained real-time observation of spermatozoa under high-magnification. Sperm selection in the IMSI group is examined at high magnification using an inverted microscope equipped with high-power differential interference contrast optics (DIC/Nomarski). The total calculated magnification is x6.600. The sperm cells exhibiting normally shaped nuclei and normal nuclear chromatin content are selected for injection.
Sponsors
Sapientiae Institute
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
FEMALE
Age
37 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Good physical and mental health * Advanced maternal age (≥ 37 years old) * Regular menstrual cycles of 25-35 days * Normal basal FSH and LH levels * Body mass index less than 30 kg/m2 * Presence of both ovaries * Intact uterus * No hormone therapy for at least 60 days preceding the study
Exclusion criteria
* Polycystic ovaries * Endometriosis * Gynaecological/medical disorders * Positive result in a screening for sexually transmitted diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Implantation rate | 6 weeks after embryo transfer | Number of embryos implanted in the uterus divided for the number of embryos transferred. |
Countries
Brazil
Outcome results
None listed