Study the Effect of Oral Zinc Supplementation on High Molecular Weight Zinc Binding Protein in Semen

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01612403

Acronym

supplement

Enrollment

Registered

2012-06-05

Start date

2011-07-31

Completion date

2012-04-30

Last updated

2012-06-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Asthenozoospermia

Keywords

zinc binding protein

Brief summary

There are several causes leading to male infertility, like oxidative stress, and nutritional deficiency of trace elements like zinc. Zinc in human seminal plasma was divided into three types of ligands which are high (HMW), intermediate (IMW), and low molecular weight ligands (LMW). The present study was conducted to study the effect of Zn supplementation on the quantitative and qualitative characteristics of semen along with Zinc Binding Protein levels in semen of patients with asthenozoospmia.

Detailed description

Elevation of ROS levels lead to an increase the oxidation of HMW-Zn binding proteins in seminal plasma of asthenozoospermic subjects. Zinc supplementation restores HMW-Zn% in seminal plasma of asthenozoospermic subjects to normal value. LMW-Zn% is elevated in seminal plasma of asthenozoospermic patients. It may be because increment the levels of semenogelin in seminal plasma of asthenozoospermic subjects. Zinc supplementation elevates LMW-Zn% in seminal plasma of asthenozoospermic subjects to more than normal value, this may be because of its enhancement of the synthesis of metallothioneins (Low molecular weight zinc binding protein).

Interventions

DIETARY_SUPPLEMENTzinc sulfate

every participant took two capsules of zinc sulfate per day for three months (each one 220mg)

Study design

Observational model

CASE_CONTROL

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

MALE

Age

27 Years to 35 Years

Healthy volunteers

Inclusion criteria

The inclusion criteria were the presence of asthenozoospermia in the semen sample.

Exclusion criteria

* the absence of endocrinopathy, * varicocele, and * female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.

Design outcomes

Primary

Measure	Time frame	Description
high molecular weight zinc binding protein	at the end of three months	elevated levels of high molecular weight zinc binding protein to normal value

Secondary

Measure	Time frame	Description
qualitative semen parameters	at the end of three months	Volume of semen, progressive sperm motility percentage and total normal sperm count increased after zinc sulfate supplementation.

Countries

Iraq

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Mar 25, 2026