Idiopathic Nephrotic Syndrome
Conditions
Keywords
Proteinuria, idiopathic, nephrotic syndrome, herpesvirus, epidemiology, immune response, interferon, steroid dependency, nephrotic disease: Idiopathic nephrotic syndrome
Brief summary
The primary purpose of the study is to identify the factors of steroid dependency in childhood idiopathic nephrotic syndrome. The steroid dependency is defined by a relapse of nephrotic syndrome within the 3 weeks that follow the withdrawal of steroid therapy after the first manifestation. Different clinical and biological factors will be analyzed: age of first manifestation, delay of remission, ethnicity, and preceding viral infection, geolocalization in the Parisian area, genoprevalence of herpes viruses and polymorphisms in the genes involved in the response to steroid therapy.
Detailed description
Patients with a steroid dependent nephrotic syndrome will be compared to those with a non steroid dependent form of nephrotic syndrome at the end of the first manifestation. An additional blood volume will be sampled in patients and controls during a scheduled biological check-up for the initial disease. Genetics factors will be checked using a DNA chips devoted to the response to steroid therapy and viral genome of EBV, CMV, HHV7 will be searched for using PCR reaction in total blood DNA extract.
Interventions
GENETICDNA chip dedicated to the pharmacogenetic of steroids
direct DNA extraction from peripheral blood cells
Sponsors
Assistance Publique - Hôpitaux de Paris
Study design
Observational model
CASE_ONLY
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
6 Months to 16 Years
Healthy volunteers
No
Inclusion criteria
* children below 16 years * first manifestation of nephrotic syndrome * steroid sensitivity according to the recommendations of the french pediatric society nephrology
Exclusion criteria
* patients who don't stay in Paris during the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| define pharmacogenetic factors of steroid dependency | 28.5 months |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| parameters of the immune response against herpesvirus, specially the cellular production of interferon induced by specific viral peptides | 28.5 months | — |
| Dynamic of the epidemiology of idiopathic nephrotic syndrome in the Paris Area | 28.5 months | Number of participants with idiopathic nephrotic syndrome in Paris Area to determinate if clusters cases exist. |
| Analysis of cortico-dependence factors | Day 1 | Comparison of polymorphisms of cortico-dependent patients with non- cortico-dependent patients |
| Analysis of susceptibility factors that generate the appearance of the Idiopathic Nephrotic Syndrome | Day 1 | Comparison of polymorphisms of all patients suffering from the Idiopathic Nephrotic Syndrome, with general population |
Countries
France
Outcome results
None listed