Vascular Endothelial Growth Factor Levels in Ectopic Pregnancy

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01601964

Acronym

VEGF

Enrollment

Registered

2012-05-18

Start date

2012-05-31

Completion date

2013-02-28

Last updated

2015-08-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Complication Following Ectopic Pregnancy

Keywords

VEGF, ectopic pregnancy.

Brief summary

The aim of this study is to determine plasma Vascular Endothelial Growth Factor level and change in patient with ectopic pregnancy who needs to surgical treatment. Patient with ectopic pregnancy diagnosed and treated with medically or surgically will be included in the study. This is an observational study.The plasma Vascular Endothelial Growth Factor level will be measured first day and two day after the diagnosis of ectopic pregnancy.

Detailed description

This study is a prospective, observational, single-center study. Ethical approval was obtained from the Institutional Review Board. This study will be completed with 30 patients with ectopic pregnancy. An intervenous cannula will be centrifuged for 15 minutes at 1000 g within 30 minutes of collection. Aliquots of plasma will be added to eppendorf tubes and stored at -20°C. Plasma Vascular Endothelial Growth Factor levels will be measured using a Human VEGF-A Platinum ELISA kit (bioscience company, Catalog No: 00122, Santa Clara, California, USA). All assays will be performed according to the manufacturer's instructions. The test kit sensitivity is 2.5 pg/ml. Intracycle variability is %4-6, intercycle variability is %8-10.

Interventions

None listed

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 46 Years

Healthy volunteers

Inclusion criteria

* Patients who suspected ectopic pregnancy

Exclusion criteria

* hemodynamically unstable patient * healthy pregnancy (a healthy increase in ß HCG) * missed abortion * incomplete abortion * The patient who need immediate surgical treatment

Design outcomes

Primary

Measure	Time frame
The primary Outcome Measure of this study is to determine plasma Vascular Endothelial Growth Factor cut-off level in patient with ectopic pregnancy who needs to surgical treatment.	3-4 months

Countries

Turkey (Türkiye)

Outcome results