Obesity
Conditions
Keywords
appetite, food, gut, brain, satiety, hunger, gut hormones
Brief summary
The primary aim of this work is, to 'relate psychological and behavioural parameters of hunger/satiety and food preference to gut hormones, neural activation and energy metabolism by dietary manipulation, across the human lifespan'.
Detailed description
The Full4Health project aims to further understanding of the mechanisms of hunger and satiety. The proposal integrates investigation of human volunteers and laboratory rodents throughout the life course, applying imaging and other cutting edge technologies to critical research questions. Full4Health will combine study of the mechanisms of hunger and satiety with intervention studies to validate the effects of the relevant food characteristics on the regulation of satiety/hunger. The development of cerebral responses to food through the gut-brain axis across lifespan particularly during childhood, adolescence and elderly will be studied.
Interventions
OTHERBreakfast Study
Participants (male and female, lean and obese, children, teenagers, adults, and elderly) will take part in 4 morning sessions, consuming a test breakfast milk based beverage and appetite. Biomarkers in blood will be measured and behavioural questionnaires completed. We will also collect a single saliva sample from each participant to examine genetic traits related to appetite, food choice, body weight, and energy expenditure. There will be two milk based beverages, one protein enriched (30% protein from calories) and one normal protein (15% protein). Participants will be offered a morning snack buffet to assess ad libitum energy intake. Phase 1 will also include a subgroup of malnourished male and female elderly participants. However, this group will only complete two morning sessions during which they will consume a low protein and a high protein milk based beverage. Appetite will be recorded and libitum energy intake will be measured. In addition, 24hr energy intake will be recorded.
OTHERfMRI Study
We will fMRI scan normal weight and overweight subjects of both gender from the four different age groups only: 8-10, 13-17, 24-45 and 65-75 years. Participants will be measured twice, on separate days, either after an overnight fast or after a test meal, fed to satiation (because hunger will modulate the response to food presentation). The participants will conduct a computerised task that will be performed in the scanner to assess hedonic responses to food cues. Physiological biomarkers will be measured during both trials for the assessment of appetite hormone circulation. Saliva samples will be taken for DNA analysis. DNA extraction techniques will be used to examine genetic traits linked to appetite, food choice, body weight, and energy expenditure.
Sponsors
European Union
University of Aberdeen
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
8 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Willingness to participate in the fMRI study * Right-handed * Not heavy smokers (less 10 day) * MRI compatibility: * no claustrophobia * no metal in the body (including dental braces)
Exclusion criteria
* Heavy smokers (more than 10/day) * Morbid obese (BMI\>40 kg/m2) * Pregnancy * Obesity of known endocrine origin * Neurological disorders including Cerebral Palsy * Alzheimers disease * Multiple Sclerosis * Parkinsons disease * Medication known to influence appetite (orlistat, oral antidiabetics, insulin, digoxin, anti-arrhythmics, sibutramine, antidepressants) * Self report fever/systemic infection * Inability to participate in fMRI scanning sessions including contraindications to MRI * Participation in medical or surgical weight loss programme within 1 month of selection * History of cerebrovascular disease * Current major depressive disorder, bipolar disorder or past history of suicide attempt or self harm * History of drug or alcohol misuse * History of significant cardiovascular disease * Allergy to any of the breakfasts components.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in concentrations of biomarkers of appetite in response to each milk-based beverage | During each of the four 'Breakfast Study' visits blood will be taken at baseline, and 30, 60, and 120 minutes after consuming the milk-based beverage. Each visit will be seperated by one week. | The following blood-borne biomarkers of appetite will be measured: * Glucose * Total cholesterol * Triglycerides * Low density lipoprotein * High density lipoprotein * Insulin * Ghrelin (active) * Glucagon-like peptide-1 (active) * Peptide YY (total) * Leptin |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Neural responses to images of food when fasted and after consuming a milk-based beverage | There will be one week between both conditions (fasted and fed) | The subjects will conduct a computerised task that will be performed in the functional magnetic resonance imaging (fMRI) scanner. Subjects will be measured twice, on separate days, either after an overnight fast or after a test meal fed to satiation. |
Countries
Greece, Netherlands, United Kingdom
Outcome results
None listed