Infertility
Conditions
Brief summary
Presently spermatozoa for ICSI are selected based on morphology and motion, without determining if acrosome reaction (AR) had occurred during sperm preparation or not. Although not critical if the sperm is injected into the ooplasm, the occurrence of AR might might be an indicator of better spermatozoon quality and implantation potential, especially in severe OTA cases. It is impossible with conventional plain optic microscopy used in IVF units to determine in vivo (without fixation) if AR has occurred, and as a result spermatozoa are injected randomly. It is readily possible to distinguish AR+ from AR- spermatozoa using polarized light microscopy due to different bifriengance. In this study the investigators seek to determine, by a prospective RCT, if selecting only acrosome reacted spermatozoa, in severe OTA cases, will improve the outcome of ICSI and the entire IVF process.
Detailed description
A prospective RCT to determine the effect of polscope based spermatozoa selection for ICSI.
Interventions
PROCEDURESperm selection by conventional / polarized light microscopy
Selecting spermatozoa for ICSI using conventional and polarized light microscopy
Sponsors
Hadassah Medical Organization
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 38 Years
Healthy volunteers
No
Inclusion criteria
* Female: normal uterine cavity and ovarian reserve. Male: TMC \< 1000000 cells.
Exclusion criteria
* Female: Abnormal uterine anatomy, low ovarian reserve, contributing egg factor, PGD.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| fertilization rate | 24-72 h | Determine the impact of AR+ spermatozoa selection of fertilization and in vitro embryonic development. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Clinical pregnancy | up to 6 weeks | The occurrence of clinical pregnancies in both arms. |
Countries
Israel
Outcome results
None listed