Aging, Disuse Atrophy
Conditions
Keywords
Immobilization, Protein supplementation
Brief summary
In the present study, the effects of 5 days of lower limb immobilization with or without twice-daily protein supplementation on muscle mass and muscle fiber characteristics will be determined. A young group will be included to compare the changes in muscle mass and muscle fiber characteristics between young and old men.
Interventions
BEHAVIORALImmobilization
Immobilization with protein (elderlY), immobilization without protein (elderly and young)
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Male * Aged from 18-35 years and from 65-75 years * 18.5 \< BMI \< 30 kg/m2
Exclusion criteria
* Smoking * Performing regular resistance training in the previous 6 months * Hypertension (according to WHO criteria) \[52\] and/or cardiovascular disease * Any back/leg/knee/shoulder complaints which may interfere with the use of crutches * Systemic use of antibiotics within 3 weeks prior to the study visit * Current systemic use of corticosteroids, growth hormone, testosterone, immunosuppressants or insulin * Type 2 diabetes mellitus * Any history of thrombosis * Any family history (1st grade) of thrombosis * All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis). * Myocardial infarction within the last 3 years * Use of anti-coagulants * Any (history of) gastrointestinal disease that interferes with GI function * Indications related to interaction with the study product: * Known allergy to milk or milk products * Known galactosemia
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in muscle mass | three days before immobilization, and directly after immobilization (day of cast removal; day 6) |
| Change in muscle fiber size | three days before immobilization, and directly after immobilization (day of cast removal; day 6) |
Secondary
| Measure | Time frame |
|---|---|
| Change in muscle strength measured by quadriceps 1-Repetition Maximum by Leg Extension | Three days before immobilization, and directly after immobilization (day of cast removal; day 6) |
Countries
Netherlands
Outcome results
None listed