Studying Genes Using Cord Blood and Placenta Samples From Relatively Healthy Newborns and Samples From Younger Patients With Wilms Tumor

Investigating the Frequency of Loss of Imprinting Across a Birth Cohort and the Link DNA Methylation Plays

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01576211

Enrollment

Registered

2012-04-12

Start date

2012-04-30

Completion date

Unknown

Last updated

2016-05-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Kidney Cancer

Keywords

Wilms tumor and other childhood kidney tumors

Brief summary

RATIONALE: Studying samples of blood and tissue from newborns and from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies cord blood and placenta tissue from newborns, and tumor tissue samples from patients with Wilms tumor.

Detailed description

OBJECTIVES: * What is the frequency of loss of imprinting at birth (in the cord blood and placenta) in a relatively healthy birth cohort? * Does deoxyribonucleic acid (DNA) methylation levels at imprinting genes have a direct association to the gene expression? OUTLINE: Archived tumor tissue, cord blood, and placenta samples are analyzed for DNA methylation, single nucleotide polymorphism, and gene expression by polymerase chain reaction (PCR), pyrosequencing, and quantitative real-time PCR. Information regarding gender and age of the samples are also collected, if possible.

Interventions

GENETICRNA analysis

GENETICDNA methylation analysis

GENETICallele-specific oligonucleotide real-time quantitative polymerase chain reaction

GENETICgene expression analysis

GENETICnucleic acid sequencing

GENETICpolymorphism analysis

OTHERlaboratory biomarker analysis

Study design

Observational model

CASE_CONTROL

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

No minimum to 21 Years

Healthy volunteers

Yes

Inclusion criteria

DISEASE CHARACTERISTICS: * Wilms tumor tissue samples from Caucasians (fresh or frozen), or DNA and ribonucleic acid (RNA) samples already isolated from patients registered on Children's Oncology Group Wilms tumor protocols * Normal/control blood samples from matched individuals * Cord blood/ placenta samples from the Michels lab Epigenetic Birth Cohort PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Design outcomes

Primary

Measure	Time frame
Frequency of loss of imprinting at birth	—
Association between methylation levels and gene expression	—

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026