Colorectal Cancer Stage 0, Colorectal Cancer Stage I, Colorectal Cancer Stage II, Colorectal Cancer Stage III, Colorectal Cancer Stage IV
Conditions
Keywords
matrilysin, MMP-7, colorectal cancer, adenoma, lymph nodes
Brief summary
Matrix metalloproteinases (MMPs) have been shown to be involved in cancer biology. Significant expression of MMP-7 (matrilysin) in colorectal cancer is mainly associated with metastatic disease even though it is expressed in most tumor states. Our purpose is to analyse MMP-7 in bowel and lymph nodes of different tumor stages and to evaluate its expression as a potential biomarker of cancer disease in patients surgically treated for benign and malignant colorectal tumors. Tumoral tissue, lymph nodes and serum samples from recruited Patients plus serum samples from healthy volunteers are analysed for matrilysin expression by histology, immunohistochemistry, ELISA and Western blotting. If Matrilysin increases with increasing dysplasia and cancer disease stage in tumor tissue as well as in the regional lymph nodes it might be used as a complement in investigating suspected locally advanced cancer.
Interventions
PROCEDUREcolonic or colorectal resection
standard laparotomic resection of colon-rectum including right hemicolectomy, left hemicolectomy, sigmoidectomy, anterior resection
Sponsors
University of Roma La Sapienza
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
40 Years to 83 Years
Healthy volunteers
Yes
Inclusion criteria
* benign colonic neoplasm not suitable for endoscopic treatment, * malignant colonic neoplasm with indication to primary surgical treatment
Exclusion criteria
* neo-adjuvant radiotherapy, * chemo-radiotherapy, * language problems and withdrawal of consent
Countries
Italy
Outcome results
None listed