Dyslipidemia, Type 2 Diabetes
Conditions
Keywords
Type 2 diabetes, Very-low-density-lipoprotein(VLDL), VLDL1, VLDL2, hyperinsulinemia, diet
Brief summary
In this study the investigators wish to compare Very Low-Density Lipoprotein (VLDL) kinetics in type 2 diabetic males and healthy males postabsorptive and during hyperinsulinemia. The kinetics is obtained using an ex-vivo VLDL1- and VLDL2-triglyceride labeling technique. The investigators hypothesis is that the investigators will find an increased VLDL1 production in type 2 diabetic males, which is not lowered by hyperinsulinemia. Also the investigators wish to investigate the influence of diet on VLDL1 and VLDL2 production in healthy males, where the investigators expect less variance in VLDL production when the subject is given an isocaloric diet.
Detailed description
Patients with type 2 diabetes are more likely to suffer from dyslipidemia. Earlier research has shown that these patients have an increased VLDL production and this could be a key factor in dyslipidemia development. Especially the production of VLDL1 seems to be increased where an inhibitory response to insulin is lacking. Further investigation in VLDL1 and VLDL2 kinetics is required. The investigators wish: 1. To extend the method of ex vivo labeling of VLDL-triglyceride, to include separate labeling of VLDL1- and VLDL2-triglyceride for evaluation of the separate lipoprotein subtype kinetics. 2. To investigate VLDL1- and VLDL2-triglyceride kinetics in the post absorptive state and under experimental hyperinsulinemia in twelve type 2 diabetic males compared to twelve healthy age, sex, and BMI matched males. 3. To investigate the effect of a three day isocaloric diet compared to a non-restricted diet on the day-to-day variation in VLDL1 and VLDL2-triglyceride kinetics in healthy men. The investigators here examine six subjects on two different days who ingest a non-restricted diet and compare this group to six subjects examined on two different days, who have prior ingest a three day isocaloric diet.
Interventions
DRUGInsulin
Insulin is given as a constant infusion( 0,6 mU/kg/min) during the hyperinsulinemia period of the study lasting four and a half hour.
DRUGinsulin
Insulin is given as a constant infusion( 0,6 mU/kg/min) during the hyperinsulinemia period of the study lasting four and a half hour.
OTHERdiet
The subjects ingest a three day isocaloric diet (55 % carbohydrate,30% fat, 15 % protein)prior to the study day.
OTHERno diet
The subjects will ingest a non-restricted diet prior to the study day.
Sponsors
University of Aarhus
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
35 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* patients with type 2 diabetes during at least six month or healthy subjects * male * BMI 25-36 kg/m2 * age 35-65
Exclusion criteria
* other diseases (hypertension and hypercholesterolemia tolerated) * Insulin treatment * alcohol abuse * smoking * medication affecting lipid metabolism apart from statins and antihypertensive drugs paused 2 weeks prior to the study day. * participation in studies involving radioactive isotopes or larger x-ray investigations within the last six month. * blood donation within three month.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| VLDL1 and VLDL2-triglyceride secretion rate (µmol/min) | 7 hours | VLDL1 and VLDL2 kinetics assessed over a single study day. |
Countries
Denmark
Outcome results
None listed