Hypercholesterolemia, Hypertension, Inflammation, Obesity, Type II Diabetes
Conditions
Keywords
anthocyanins, antioxidant, polyphenols, bioavailability, cholesterol, insulin sensitivity, glucose tolerance, atherosclerosis, metabolic syndrome
Brief summary
The hypothesis is that richly coloured purple vegetables, rich in polyphenolic compounds including anthocyanins will have higher antioxidant and other biological activities, than more lightly coloured versions of these foods. Diets of human subjects will be modified to allow consumption of 200-300 g of raw carrots or cooked potatoes. Participants will be randomized to consume either orange or purple carrots, or white or purple potatoes. They will consume these diets for 12 weeks and bioavailability of polyphenolics will be examined as well as anthropometry and blood biochemistry for changes in risk factors associated with cardiovascular disease.
Interventions
OTHERvegetable
200-300 g raw carrots or 300-500 g cooked potatoes
Sponsors
Ontario Ministry of Agriculture, Food and Rural Affairs
University of Guelph
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* adult men and women 18-65 years of age * must have a least one of the following risk factors associated with increased risk of type II diabetes and/or cardiovascular disease: * borderline high or hypertension or undergoing treatment for such * abnormal fasting blood glucose or undergoing treatment for such * overweight or obese * borderline high or high LDL-cholesterol or undergoing treatment for such * borderline low or low HDL-cholesterol * borderline high or high triglycerides or undergoing treatment for such
Exclusion criteria
* smokers, pregnant or nursing women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood cholesterol | 12 weeks | blood will be collected at baseline before the experimental foods are introduced and again 6 weeks into the intervention period and during the last week of the intervention (week 12) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| blood pressure | 12 weeks | Participants will have their body weight and blood pressure taken at weekly counselling sessions |
| body composition | 12 weeks | body composition will be measured in well hydrated subjects using bioelectric impedence analysis |
| insulin resistance | 12 weeks | insulin resistance will be measured by way of an oral glucose tolerance test and collection of venous blood samples for the subsequent 3 hours on two occasions, baseline and during the last week of the intervention diet. |
| blood and urinary polyphenol metabolites | 12 weeks | venous blood and urine will be collected to determine the metabolic profiles of polyphenolics in individual participants. Based on intake data, the bioavailability of the polyphenols in the foods will be estimated. |
| circulating biomarkers of cardiovascular disease and type II diabetes risk | 12 weeks | Blood will be collected for measurement of multiple cytokines, growth factors and other blood biomarkers associated with these diseases. |
Countries
Canada
Contacts
Primary ContactKelly A Meckling, PhD
Backup ContactSaqib Mannan, BSc
Outcome results
None listed