Streptococcal Pharyngitis
Conditions
Keywords
Streptococcus pyogenes, Streptococcus
Brief summary
The purpose of the research is to help understand why some children become carriers of strep and whether children who are carriers need to be treated with antibiotics.
Detailed description
The overall objective of this investigation is to understand the differences in Group A streptococci in children who are acutely infected from those who are carriers. The hypothesis is that when in the carrier state, GAS exhibits unique transcriptional profiles that differ from those of the acute infection state. The investigators expect transcriptional profiles of GAS to provide important information regarding the changes the organism undergoes when transitioning between acute infection and carriage. The specific aims of this study are: 1. To collect longitudinal participant-samples from acute and carriage phases of GAS infection and compare transcriptomic profiles and whole genome sequences of GAS recovered from acute and carrier pharyngeal swabs obtained from the same participants. 2. To evaluate how identified differentially expressed genes, or observed genetic polymorphisms, influence GAS models of bacterial colonization and pathogenesis. To do this, the investigators will to identify 12 children with acute pharyngitis due to Group A streptococcus (GAS) who are pharyngeal carriers of GAS. Thirty percent of children 4 to 16 years of age with acute pharyngitis occurring between October and May will have a positive culture or rapid antigen detection test for GAS. Approximately 8-10% of these children with pharyngitis and a positive culture or rapid antigen detection test (RADT) for GAS will be carriers. Therefore,180 participants will need to be enrolled.
Interventions
OTHERIdentifying group A strep carriers
At study entry, at 14 days, and if follow up is positive, again in 14-21 days: Standard culture for GAS and analysis of mRNA.
Sponsors
University of Wisconsin, Madison
National Institute of Allergy and Infectious Diseases (NIAID)
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
5 Years to 15 Years
Healthy volunteers
No
Inclusion criteria
* Children ages 5-15 years * Positive rapid antigen detection test for group A streptococcus * Parent or legal guardian present and able to provide consent * Provider prescribes treatment with a beta lactam antibiotic * English speaking
Exclusion criteria
* Unable to take beta lactam antibiotics * Other concurrent bacterial infection, i.e., pneumonia
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Identifying children who are pharyngeal carriers of Group A streptococcus | 2 weeks | The specific aim of this study is to identify 30 children with acute pharyngitis due to Group A streptococcus (GAS) and 30 children who are pharyngeal carriers of GAS. Thirty percent of children 4 to 16 years of age with acute pharyngitis occurring between October and May will have a positive culture or rapid antigen detection test for GAS. Approximately 10-15% of these children with pharyngitis and a positive culture or rapid antigen detection test (RADT) for GAS will be carriers |
Countries
United States
Contacts
CONTACTCherie Schommer, BA
PRINCIPAL_INVESTIGATORGregory DeMuri, MD
University of Wisconsin, Madison
Outcome results
None listed