Infertility
Conditions
Brief summary
Cytoplasmatic maturation is an important event in the general maturation of of the oocyte. Mitochondria is one of the major organelles involved in the process of cytoplasmatic maturation. The aim of this study is to investigate the presence and distribution of the mitochondria in the cytoplasma of immature oocytes that are retrieved in the routine in-vitro maturation cycle with intracytoplasmatic sperm injection (ICSI). These oocytes will not undergo ICSI because of their immaturity. Usually, they are discarded. In addition to these immature oocytes the investigators shall also investigate the mitochondria in mature oocytes that underwent ICSI but did not become fertilized and are discarded.
Interventions
OTHERFixation and staining
Fixation and staining by MitiTracker Green FM
OTHERObservation by laser microscopy
Observation with Laser scanning confocal microscopy
Sponsors
Hillel Yaffe Medical Center
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 44 Years
Healthy volunteers
No
Inclusion criteria
* Infertile patients needing IVF treatment
Exclusion criteria
* Patients not requiring the ICSI procedure
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The patterns of mitochondrial distribution within the cytoplasma of oocytes during nuclear maturation | Three months | Identification the different patterns of mitochondrial distribution in correlation with the 3 stages nuclear maturation in human oocytes |
Countries
Israel
Contacts
Primary ContactAdrian Ellenbogen, MD
Outcome results
None listed