Leukemia
Conditions
Keywords
adult acute myeloid leukemia, childhood acute myeloid leukemia/other myeloid malignancies
Brief summary
RATIONALE: Studying samples of blood, tissue, and bone marrow from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies the role of biomarkers in disease progression in samples from patients with acute myeloid leukemia.
Detailed description
OBJECTIVES: * To determine the subcellular localization of the MLL-AF1q fusion protein in leukemia cells. OUTLINE: Cryopreserved specimens are analyzed for MLL-AF1q fusion protein and AF1 gene expression by mass spectrometry, western blot analysis, semi-quantitative PCR, and qPCR.
Interventions
GENETICgene expression analysis
GENETICgene rearrangement analysis
GENETICpolymerase chain reaction
GENETICprotein analysis
GENETICwestern blotting
OTHERlaboratory biomarker analysis
OTHERmass spectrometry
Sponsors
National Cancer Institute (NCI)
Children's Oncology Group
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 30 Years
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Cryopreserved acute myeloid leukemia specimens PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Subcellular localization of the MLL-AF1q fusion protein in leukemia cells | — |
Outcome results
None listed