Insulin Resistance
Conditions
Keywords
Insulin resistance, Abdominal obesity, Calorie restriction
Brief summary
This study is being done to understand the effects of calorie restriction and weight loss on muscle protein metabolism in adult men and women.
Interventions
BEHAVIORALCalorie Restriction
Participants will work with the CRU dietetics staff to plan a diet that will result in their losing 1-2 pounds per week over 16 weeks. They will wear an accelerometer to track there activity during these weeks.
BEHAVIORALControl (normal living)
Participants will continue their normal living for entire time of the study. They will be asked to wear an accelerometer for the next 16 weeks and check in weekly at the CRU to exchange accelerometers.
Sponsors
Mayo Clinic
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
45 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * BMI will be between 30 kg/m2 - 38 kg/m2 and waist circumference for women ≥ 88 cm and men ≥ 104 cm * Age 45 to 65 years. * Male and Female
Exclusion criteria
* Active coronary artery disease * BMI \< 30 kg/m2 Participation in structured exercise (\>2 times per week for 30 minutes or longer) * Smoking * Medications known to affect muscle metabolism (beta blockers) * Renal failure (serum creatinine \> 1.5mg/dl) * Chronic active liver disease (AST and ALT \> 3 times normal) * Fasting blood glucose \> 126 mg/dl * Anti-coagulant therapy (warfarin/heparin)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in muscle protein synthesis from baseline to 16 weeks | Measured at week 0 (baseline) and week 16 | The investigators will determine the rate of incorporation of stable isotope amino acid tracers in skeletal muscle protein on average resting muscle protein synthesis rate (% new muscle protein per hour) and will be performed at baseline and 16 weeks after caloric restriction or control diet. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in insulin sensitivity from 0 (baseline) and 16 weeks | Measured at week 0 (baseline) and week 16 | The investigators will determine insulin sensitivity at week 0 (baseline) and 16 weeks into caloric restriction or control diet. The measurement is made by hyperinsulimic euglycemic clamp and reported as μmol per kilogram of fat free mass of infused glucose needed to maintain glycemia. |
Countries
United States
Outcome results
None listed