Respiratory Inflammation, Systemic Inflammation
Conditions
Keywords
Wood smoke, inflammation, lung function, arterial stiffness, heart rate variability, Respiratory and systemic inflammation following exposure to woodsmoke in healthy volunteers
Brief summary
Air pollution is linked to respiratory and cardiovascular disease. Wood smoke is a common air in many parts of the world and previous studies indicate that wood smoke induce oxidative stress in the respiratory tract. It is not determined how different types of biomass combustion affect human health. In this study the investigators plan to investigate how inhalation of wood smoke and pellets smoke affect respiratory and cardiovascular health.
Interventions
OTHERBronchoscopy
24 hours after exposure, a bronchoscopy will be performed to allow analysis of cells and inflammatory markers in bronchial wash, bronchioalveolar lavage and lung biopsies.
Sponsors
Umeå University
University of Edinburgh
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy volunteers
Exclusion criteria
* Use of regular medication except the oral contraceptive pill * Current smokers * Significant occupation exposure to air pollution * Intercurrent illness
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Respiratory inflammation | 24 hours after exposure to air or woodsmoke | Bronchoscopy will be performed, and samples collected by bronchoalveolar lavage and bronchial biopsy. Samples will be analysed for cell counts, histology and inflammatory markers. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Central arterial stiffness | Baseline and for 1 hour after exposure | Central arterial stiffness will be measured at baseline and for the 1 hour following the exposure using the SphygmoCor and Vicorder devices |
| Heart rate variability | 24 hours after the exposure | Continuous electrocardiograms will be recorded using a holter monitor to determine heart rate variability |
| Systemic inflammation | 24 hours after the exposure | Blood samples will be taken at intervals following the exposure to woodsmoke to measure circulating inflammatory markers |
| Lung function | Baseline and 24hrs after exposure | Lung function will be assessed using spirometry before and after each exposure |
Countries
Sweden
Outcome results
None listed