Men
Conditions
Keywords
Dietary protein, Canola protein, Protein metabolism, Protein-enriched drinks, Nitrogen balance
Brief summary
The objective of the study is to evaluate the nutritional and physiological properties of two canola proteins focusing on the bioavailability in humans.
Detailed description
The physiological effects of a single administration of two different industrially manufactured canola proteins (hydrolyzate, isolate) were investigated compared to soy protein. Twenty-eight healthy men (ø 25 years) completed a double-blind, placebo-controlled, crossover study. The subjects were randomly divided into two groups (group A: canola protein isolate and soy protein isolate and group B: canola protein hydrolyzate and soy protein isolate). After a three-day run-in period, on the intervention day, the subjects consumed a protein drink containing 30.0 g protein powder added to a final volume of 500 mL tomato juice. Half of the subjects in each group consumed a protein drink containing 30.0 g canola protein, the other half received the soy protein. After taking of fasting blood samples in the morning of the intervention day, blood samples were drawn at regular intervals for a period of eight hours. At the end of the run-in period and on the intervention day, a 24-hour urine collection took place. Within a second experiment (after 4 weeks), the protein source was crossed within the four subgroups for the second intervention.
Interventions
DIETARY_SUPPLEMENTProtein drink - protein isolate
The subjects consumed 500 mL of a protein drink (tomato juice) containing 30 g protein (canola protein isolate or reference protein) after overnight fasting. After run-in period, each volunteer have to pass both protein-intervention days in different order with a four-week wash out period in between.
DIETARY_SUPPLEMENTProtein drink - protein hydrolyzate
The subjects consumed 500 mL of a protein drink (tomato juice) containing 30 g protein (canola protein hydrolyzate or reference protein) after overnight fasting. After run-in period, each volunteer have to pass both protein-intervention days in different order with a four-week wash out period in between.
Sponsors
University of Jena
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
SCREENING
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
20 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Nonsmoker * Body mass index: 20-26 kg/m²
Exclusion criteria
* Intake of pharmaceuticals * Allergy against canola protein or soy protein * Kidney disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma amino acids | At Day 1 (Intervention day); After 0, 30, 60, 90, 120, 180, 240, 300, 360, 420 and 480 minutes | Free proteinogenic amino acids |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Body composition (body status) | At day 1 | Bioelectrical impedance analysis, body weight |
| Nitrogen balance | After day 0 and 1 | Nitrogen balance = nitrogen intake - nitrogen output (urine nitrogen + fecal nitrogen + skin nitrogen); Nitrogen intake was calculated from food uptake; Urine nitrogen contains urea, uric acid, creatinine and total protein; Fecal nitrogen was calculated as the tenth part of the nitrogen intake; Skin nitrogen was calculated as 5.00 mg per kg of body weight |
Countries
Germany
Outcome results
None listed