Infertility
Conditions
Keywords
coculture, endometrial biopsy, IVF poor responders
Brief summary
The purpose of this study is to determine if the use of Autologous Endometrial Coculture (AECC) in young in vitro fertilization (IVF) patients with poor ovarian reserve improves pregnancy outcome.
Detailed description
Young women with poor ovarian reserve are commonly given a poor prognosis for IVF success. Standard culture media for fertilizing oocytes (unfertilized eggs) is commonly used in IVF which closely resembles tubal fluid. Efforts to create optimal culture conditions for fertilization of human embryos for women with poor quality embryos and/or repeated implantation failures have been developed at our center by using AECC. AECC involves placing a patient's fertilized eggs on top of a layer of cells from her own uterine lining, creating a more natural environment for embryo development and maximizing the chance for IVF pregnancy. The investigators will prospectively randomize young patients (≤ age 38 years) poor ovarian reserve to AECC or no AECC with standard culture media to study pregnancy outcome.
Interventions
PROCEDUREAECC (Autologous endometrial coculture)
endometrial biopsy with autologous endometrial coculture
OTHERno endometrial biopsy or Autologous endometrial coculture (AECC) performed
routine procedures with out biopsy
Sponsors
Weill Medical College of Cornell University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 38 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy women undergoing IVF at CRMI * Age ≤ 38 years * Poor ovarian reserve with antral follicle count less than 6 follicles, cycle day 2/3 FSH \> 12 ng/ml, AMH \< 0.5 ng/ml
Exclusion criteria
* Healthy women undergoing IVF with male factor, severe endometriosis, or tubal factor infertility * Donor patients * Contraindications for the use of gonadotropins (i.e. pregnancy, lactation, undiagnosed vaginal bleeding or ovarian cysts) * Recent or current medical conditions where the patient is not medically stable to undergo stimulation or egg retrieval, HIV infection, diabetes, cardiovascular disease, gastrointestinal, hepatic disease, renal or pulmonary disease. * Any patient who is not a candidate for IVF
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Pregnancy outcome | 1 month |
Secondary
| Measure | Time frame |
|---|---|
| Embryo quality | 1 month |
Countries
United States
Outcome results
None listed