Induced Pluripotent Stem Cells
Conditions
Brief summary
Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.
Detailed description
Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.
Interventions
OTHERgeneration of stem cells
Collected germ cells will be cultured in medium. The germ cell cultures will be infected with reprograming factors. Rare colonies with characteristic stem cell morphologies will be expanded.
Sponsors
Rambam Health Care Campus
Study design
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
1. males over the age of 18 and under 50 2. no known chronic illness 3. not taking any chronic drugs
Exclusion criteria
1. females 2. males under 18 or over 50 3. any known chronic illness 4. taking chronic medications
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Patients donated samples | June 2011-November 2013 | No samples collected to date |
Secondary
| Measure | Time frame |
|---|---|
| No secomdary outcome | June 2011 - November 2013 |
Countries
Israel
Outcome results
None listed