Does Nesiritide Provide Renal Protection

Does Nesiritide Provide Renal Protection By Attenuating the Inflammatory Response

Status

Completed

Phases

Phase 2

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT01440881

Enrollment

Registered

2011-09-27

Start date

2010-04-30

Completion date

2012-12-31

Last updated

2014-06-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Renal Failure

Keywords

renal failure, cardiopulmonary bypass

Brief summary

This project evaluates whether nesiritide, human recombinant brain natriuretic peptide, confers renal protection during cardiovascular surgery by attenuating the inflammatory response.

Detailed description

This will be a randomized, prospective blinded pilot study comparing nesiritide to placebo in patients undergoing aortic arch, ascending aorta, aortic valve or mitral valve surgery with baseline GFR 30-90ml/min. Patients were randomized to either placebo or nesiritide (administered prophylactically starting in the operating room prior to incision at 0.01 mcg/kg /minute with no bolus and continued for 48 hours).

Interventions

DRUGNesiritide

infuses at 0.01MCG/KG/min for 48 hours

DRUGPlacebo

infuses at 0.01MCG/KG/min for 48 hours

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

PREVENTION

Masking

TRIPLE (Subject, Caregiver, Investigator)

Eligibility

Sex/Gender

ALL

Age

18 Years to 80 Years

Healthy volunteers

Inclusion criteria

* Patients undergoing surgery on their aortic arch, ascending aorta, or having aortic valve or mitral valve surgery * Baseline MDRD (modification of diet in renal disease) calculated GFR (Glomerular Filtration Rate) 30-90 ml/min. * Signed Informed Consent

Exclusion criteria

* Age \<18 or \>80 * Ejection Fraction \<30%. * Presence of endocarditis or other infection. * Presence or anticipated use of an intra-aortic balloon pump. * History of an organ transplant. * History of an adverse reaction to nesiritide. * Surgery performed without cardiopulmonary bypass. * Receiving Aprotinin * Dopamine administered in doses \<5 mcg /kg min. * Women of child bearing potential or pregnant or breastfeeding. * Participation in any other investigational trial * Jehovah Witness * Bleeding Disorder * Active Endocarditis

Design outcomes

Primary

Measure	Time frame	Description
Measure Neutrophils to Measure Kidney Injury	30 days from the start of infusion	0.35 mL of whole blood from each patient was applied to a microfluidics chip to isolate neutrophils. Total RNA was subsequently isolated and gene expression (for all genes listed below) was measured with an Affymetrix gene chip. Data was normalized using RMA (Robust multi-array average) and expressed as log2 expression.
The Examine and Measure Cytokines to Measure Kidney Injury	30 days from the start of infusion	Serial measurement of serum cytokine profiles were measured with multiplex Luminex plates that enable the simultaneous measurement of 23 cytokines.
The Examine and Measure Endothelin-1 to Measure Kidney Injury	30 days from the start of infusion	Serial measurements of Endothelin-1 levels were measured to determine if natriuretic peptides exert their renal protective effects by preserving renal afferent arteriole flow by antagonizing the vasoconstrictive effects of Endothelin-1.
The Examine and Measure Urinary NGAL to Measure Kidney Injury	A change in urinary NGAL 2 hours after bypass was stopped and 5 minutes after the start of spontaneous circulation was resumed.	Urinary NGAL,a biomarker for kidney injury was measured.

Countries

United States

Participant flow

Recruitment details

Starting in June 2010 and ending in December 2012, subjects were seen in outpatient clinics or in the hospital. The pre-operative labs were used to screen and enroll subjects.

Pre-assignment details

Out of the 37 signed informed consent there were 8 subjects who were consented and did not participate: 2 subjects changed their mind, 5 subjects creatinine level was not within the protocol level for participation, 1 subject surgery was canceled. This left 29 subjects to participate in the study.

Participants by arm

Arm	Count
Nesiritide infuses at 0.01 MCG (micrograms)/KG (kilograms)/min (minute) for 48 hours Nesiritide: infuses at 0.01MCG/KG/min for 48 hours	14
Placebo infuses at 0.01MCG/KG/min for 48 hours Placebo: infuses at 0.01MCG/KG/min for 48 hours	15
Total	29

Withdrawals & dropouts

Period	Reason	FG000	FG001
Overall Study	Death	0	1

Baseline characteristics

Characteristic	Nesiritide	Placebo	Total
Age, Categorical <=18 years	0 Participants	0 Participants	0 Participants
Age, Categorical >=65 years	10 Participants	6 Participants	16 Participants
Age, Categorical Between 18 and 65 years	4 Participants	9 Participants	13 Participants
Region of Enrollment United States	14 participants	15 participants	29 participants
Sex: Female, Male Female	5 Participants	5 Participants	10 Participants
Sex: Female, Male Male	9 Participants	10 Participants	19 Participants

Adverse events

Event type	EG000 affected / at risk	EG001 affected / at risk
deaths Total, all-cause mortality	— / —	— / —
other Total, other adverse events	2 / 14	1 / 15
serious Total, serious adverse events	0 / 14	3 / 15

Outcome results

Primary

Measure Neutrophils to Measure Kidney Injury

0.35 mL of whole blood from each patient was applied to a microfluidics chip to isolate neutrophils. Total RNA was subsequently isolated and gene expression (for all genes listed below) was measured with an Affymetrix gene chip. Data was normalized using RMA (Robust multi-array average) and expressed as log2 expression.

Time frame: 30 days from the start of infusion

Arm	Measure	Group	Value (MEAN)	Dispersion
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: PRSS3	6.338 log2 expression	Standard Deviation 0.123
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: IL23A	4.508 log2 expression	Standard Deviation 0.177
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: C6orf145	5.501 log2 expression	Standard Deviation 0.216
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: HIST1H1C	8.888 log2 expression	Standard Deviation 0.506
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: TBC1D26 /// ZNF286A	4.990 log2 expression	Standard Deviation 0.092
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: RPL27	9.269 log2 expression	Standard Deviation 0.191
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC440087	3.553 log2 expression	Standard Deviation 0.181
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: OXT	6.899 log2 expression	Standard Deviation 0.175
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: GIPC3	6.158 log2 expression	Standard Deviation 0.124
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: FAM98C	6.778 log2 expression	Standard Deviation 0.146
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: PTOV1	8.234 log2 expression	Standard Deviation 0.21
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: RPS16	9.077 log2 expression	Standard Deviation 0.195
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: ERCC1	5.798 log2 expression	Standard Deviation 0.171
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC339344	7.522 log2 expression	Standard Deviation 0.238
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: ATG10	5.347 log2 expression	Standard Deviation 0.39
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: SLC35C1	6.422 log2 expression	Standard Deviation 0.155
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: PTPMT1 /// NDUFS3 /// PTPMT1	6.702 log2 expression	Standard Deviation 0.168
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: SIDT2	6.792 log2 expression	Standard Deviation 0.215
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: Q6ZVY8_HUMAN	5.229 log2 expression	Standard Deviation 0.124
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: CECR5	5.519 log2 expression	Standard Deviation 0.108
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: VPS35	9.042 log2 expression	Standard Deviation 0.339
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LGALS2	5.513 log2 expression	Standard Deviation 0.174
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: NDUFA6	6.496 log2 expression	Standard Deviation 0.334
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC497190 /// NP_001011880.1	6.469 log2 expression	Standard Deviation 0.089
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: CXorf52	6.151 log2 expression	Standard Deviation 0.48
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: MAP4K4	10.656 log2 expression	Standard Deviation 0.121
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC441241 /// VKORC1L1	6.418 log2 expression	Standard Deviation 0.349
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: DBF4	4.706 log2 expression	Standard Deviation 0.245
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: SP100	9.074 log2 expression	Standard Deviation 0.242
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: TPT1	9.177 log2 expression	Standard Deviation 0.139
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC285908	7.517 log2 expression	Standard Deviation 0.173
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOXHD1	6.886 log2 expression	Standard Deviation 0.336
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: DYM	8.158 log2 expression	Standard Deviation 0.241
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: Q6ZU44_HUMAN	4.953 log2 expression	Standard Deviation 0.2
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: BAZ2B	8.802 log2 expression	Standard Deviation 0.33
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: GRM8	4.303 log2 expression	Standard Deviation 0.114
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: Q6ZP42_HUMAN	4.967 log2 expression	Standard Deviation 0.251
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: HTR2B	4.337 log2 expression	Standard Deviation 0.263
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: TUFT1	5.453 log2 expression	Standard Deviation 0.251
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC728449 /// ANXA8L2	5.755 log2 expression	Standard Deviation 0.105
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: RPS24	7.029 log2 expression	Standard Deviation 0.153
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: DOLPP1	5.707 log2 expression	Standard Deviation 0.218
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: UBQLN1	9.129 log2 expression	Standard Deviation 0.174
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: TNFSF15	5.080 log2 expression	Standard Deviation 0.296
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: Q5QFB9_HUMAN	4.770 log2 expression	Standard Deviation 0.456
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: CYP19A1	3.696 log2 expression	Standard Deviation 0.126
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: Q6UWF5_HUMAN	3.851 log2 expression	Standard Deviation 0.309
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: LOC340204 /// NP_001010886.1	4.453 log2 expression	Standard Deviation 0.208
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: CCDC76	3.702 log2 expression	Standard Deviation 0.211
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: PRAMEF8	5.828 log2 expression	Standard Deviation 0.232
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: RPL26	7.440 log2 expression	Standard Deviation 0.205
Nesiritide	Measure Neutrophils to Measure Kidney Injury	Gene: ZNF540	4.284 log2 expression	Standard Deviation 0.11
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: MAP4K4	10.651 log2 expression	Standard Deviation 0.159
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: TUFT1	5.630 log2 expression	Standard Deviation 0.195
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: PRSS3	6.241 log2 expression	Standard Deviation 0.118
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: IL23A	4.546 log2 expression	Standard Deviation 0.301
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC441241 /// VKORC1L1	6.533 log2 expression	Standard Deviation 0.453
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: C6orf145	5.444 log2 expression	Standard Deviation 0.156
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: PRAMEF8	5.856 log2 expression	Standard Deviation 0.233
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: HIST1H1C	9.404 log2 expression	Standard Deviation 0.903
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: DBF4	4.776 log2 expression	Standard Deviation 0.364
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: TBC1D26 /// ZNF286A	4.930 log2 expression	Standard Deviation 0.113
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC728449 /// ANXA8L2	5.801 log2 expression	Standard Deviation 0.195
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: RPL27	9.315 log2 expression	Standard Deviation 0.214
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: SP100	9.205 log2 expression	Standard Deviation 0.191
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC440087	3.647 log2 expression	Standard Deviation 0.218
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: CYP19A1	3.725 log2 expression	Standard Deviation 0.131
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: OXT	6.884 log2 expression	Standard Deviation 0.102
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: TPT1	9.242 log2 expression	Standard Deviation 0.125
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: GIPC3	6.097 log2 expression	Standard Deviation 0.114
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: ZNF540	4.255 log2 expression	Standard Deviation 0.12
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: RPS24	7.063 log2 expression	Standard Deviation 0.153
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: FAM98C	6.857 log2 expression	Standard Deviation 0.129
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC285908	7.454 log2 expression	Standard Deviation 0.322
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: PTOV1	8.153 log2 expression	Standard Deviation 0.149
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: CCDC76	3.627 log2 expression	Standard Deviation 0.211
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: RPS16	9.012 log2 expression	Standard Deviation 0.221
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOXHD1	6.742 log2 expression	Standard Deviation 0.398
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: ERCC1	5.843 log2 expression	Standard Deviation 0.242
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: DOLPP1	5.759 log2 expression	Standard Deviation 0.2
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC339344	7.502 log2 expression	Standard Deviation 0.164
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: DYM	8.088 log2 expression	Standard Deviation 0.281
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: ATG10	5.286 log2 expression	Standard Deviation 0.308
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: Q6UWF5_HUMAN	3.893 log2 expression	Standard Deviation 0.209
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: SLC35C1	6.358 log2 expression	Standard Deviation 0.188
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: Q6ZU44_HUMAN	4.751 log2 expression	Standard Deviation 0.159
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: PTPMT1 /// NDUFS3 /// PTPMT1	6.706 log2 expression	Standard Deviation 0.177
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: UBQLN1	9.172 log2 expression	Standard Deviation 0.214
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: SIDT2	6.870 log2 expression	Standard Deviation 0.161
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: BAZ2B	8.811 log2 expression	Standard Deviation 0.117
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: Q6ZVY8_HUMAN	5.132 log2 expression	Standard Deviation 0.214
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: RPL26	7.481 log2 expression	Standard Deviation 0.212
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: CECR5	5.499 log2 expression	Standard Deviation 0.105
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: GRM8	4.259 log2 expression	Standard Deviation 0.142
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: VPS35	8.974 log2 expression	Standard Deviation 0.379
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: TNFSF15	5.072 log2 expression	Standard Deviation 0.33
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LGALS2	5.565 log2 expression	Standard Deviation 0.187
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: Q6ZP42_HUMAN	5.040 log2 expression	Standard Deviation 0.336
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: NDUFA6	6.680 log2 expression	Standard Deviation 0.455
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC340204 /// NP_001010886.1	4.437 log2 expression	Standard Deviation 0.273
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: LOC497190 /// NP_001011880.1	6.470 log2 expression	Standard Deviation 0.116
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: HTR2B	4.037 log2 expression	Standard Deviation 0.289
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: CXorf52	6.301 log2 expression	Standard Deviation 0.387
Placebo	Measure Neutrophils to Measure Kidney Injury	Gene: Q5QFB9_HUMAN	4.430 log2 expression	Standard Deviation 0.427

Primary

The Examine and Measure Cytokines to Measure Kidney Injury

Serial measurement of serum cytokine profiles were measured with multiplex Luminex plates that enable the simultaneous measurement of 23 cytokines.

Time frame: 30 days from the start of infusion

Population: randomized per protocol

Arm	Measure	Group	Value (MEDIAN)
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 1 hour post bypass	124.298 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 time point 0	28.167 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 1 hour post bypass	33.457 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 2 hour post bypass	44.383 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 4hour post bypass	35.759 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 6 hour post bypass	28.593 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 8 hours post bypass	29.794 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 24 hours post bypas	29.513 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 baseline	5.377 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8- 1 hour post bypass	17.107 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8-2 hour post bypass	17.068 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 4 hour post bypass	16.005 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 6 hour post bypass	14.290 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 8hour post bypass	14.550 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 24 hour post bypass	12.264 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL10 baseline	8.063 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL10 0 time point	1289.776 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 1 hour post bypass	1558.903 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL10 2 hour post bypass	667.765 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 4 hour post bypass	309.351 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 6 hour post bypass	107.778 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 8 hour post bypass	103.791 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 24 hour post bypass	18.597 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha baseline	6.286 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 0 time point	8.253 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 1hour post bypass	10.729 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 2 hour post bypass	9.855 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 4 hour post bypass	8.843 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 6 hour post bypass	7.185 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 8 hour post bypass	5.175 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 24 hour post bypass	4.620 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta baseline	1.162 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 0 time point	1.110 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 4 hour post bypass	1.698 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 1 hour post bypass	0.898 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 2 hour post bypass	1.302 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 6 hour post bypass	1.234 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 8 hour post bypass	1.389 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 24 hour post bypass	0.309 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF baseline	102.054 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 0 time point	70.647 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 1 hour post bypass	89.814 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 2 hour post bypass	91.881 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 6 hour post bypass	83.581 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 8 hour post bypass	84.494 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 24 hour post bypass	76.771 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 baseline	174.848 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 0 timepoint	478.742 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 1 hour post bypass	483.095 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 2 hour post bypass	441.203 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 4 hour post bypass	265.736 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 6 hour post bypass	191.735 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 8 hour post bypass	180.749 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 24 hour post bypass	202.621 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF baseline	47.564 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 0 timepoint	31.242 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 1 hour post bypass	35.745 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 2 hour post bypass	24.052 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 4 hour post bypass	29.841 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 6 hour post bypass	33.050 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 8 post bypass	27.022 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 24 hour post bypass	30.494 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha baseline	12.372 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 0 time	41.216 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 1 hour post bypass	26.101 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 2 hour post bypass	22.122 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 4 hour post bypass	18.137 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 6 hour post bypass	15.148 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 8 hour post bypass	9.334 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 24 hour post bypass	2.974 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta baseline	1.014 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 0 time point	0.775 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 1 hour post bypass	0.653 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 2 hour post bypass	1.311 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 4 hour post bypass	1.716 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 6 hour post bypass	1.119 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 8 hour post bypass	0.875 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 24 hour post bypass	0.000 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA baseline	26.419 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 0 time point	41.945 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 1 hour post bypass	68.792 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 2 hour post bypass	98.089 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 4 hour post bypass	105.861 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 6 hour post bypass	67.705 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 8 hour post bypass	45.097 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 24 hour post bypass	21.181 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 baseline	0.850 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 0 timepoint	1.130 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 1 hour post bypass	1.714 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 2 hour post bypass	1.714 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 4 hour post bypass	1.753 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 6 hour post bypass	1.297 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 8 hour post bypass	1.281 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 24 hour post bypass	0.921 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 baseline	0.093 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 0 timepoint	0.185 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 1 hour post bypass	0.000 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 2 hour post bypass	0.000 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 4 hour post bypass	0.000 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL_3 6 hour post bypass	0.061 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 8 hour post bypass	0.061 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL_3 24 hour post bypass	0.073 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 baseline	4.295 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 0 timepoint	6.285 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 1 hour post bypass	6.221 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL_4 2 hour post bypass	5.534 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 8 hour post bypass	22.676 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 24 hour post bypass	18.726 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF baseline	50.953 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 0 time point	92.559 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 2 hour post bypass	249.816 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 4 hour post bypass	285.277 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 6 hour post bypass	252.546 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 8 hour post bypass	264.061 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 24 hour post bypass	309.690 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 4 hour post bypass	6.743 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 6 hour post bypass	4.859 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 8 hour post bypass	4.063 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL_4 24 hour post bypass	3.634 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 baseline	0.563 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 0 timepoint	0.134 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 1 hour post bypass	0.169 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 2 hour post bypass	0.291 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 4 hour post bypass	0.626 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 6 hour post bypass	0.334 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 8 hour post bypass	0.387 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 24 hour post bypass	0.373 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 baseline	4.032 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 0 timepoint	3.673 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 1 hour post bypass	2.973 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 2 hour post bypass	2.469 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 4 hour post bypass	3.061 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 6 hour post bypass	3.391 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 8 hour post bypass	3.506 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 24 hour post bypass	3.656 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 baseline	0.922 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 0 timepoint	0.133 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 4 hour post bypass	1.571 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 6 hour post bypass	1.138 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 8 hour post bypass	0.786 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 24 hour post bypass	0.094 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 baseline	1.905 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 0 timepoint	3.200 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 1 hour post bypass	4.228 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 2 hour post bypass	5.556 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 4 hour post bypass	5.723 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 6 hour post bypass	5.021 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 8 hour post bypass	4.984 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 24 hour post bypass	6.120 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 baseline	1.171 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 0 time point	0.765 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 1 hour post bypass	2.174 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 2 hour post bypass	1.381 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 4 hour post bypass	1.682 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 6 hour post bypass	0.815 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL- 17 8 hour post bypass	0.817 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 24 hour post bypass	0.397 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 baseline	506.657 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 0 time point	2340.935 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 1 hour post bypass	1828.152 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 2 hour post bypass	1571.877 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 4 hour post bypass	1072.023 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 6 hour post bypass	704.208 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 8 hour post bypass	448.938 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 24 hour post bypass	243.332 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha baseline	2.251 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 0 time point	8.880 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 1 hour post bypass	9.042 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 2 hour post bypass	6.610 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 4 hour post bypass	4.024 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 6 hour post bypass	3.017 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 8 hour post bypass	1.933 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 24 hour post bypass	1.279 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta baseline	22.626 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 1 hour post bypass	85.082 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 2 hour post bypass	53.750 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 4 hour post bypass	34.010 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-8 0 time point	16.821 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 4 hour poswt bypass	88.005 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 1 hour post bypass	0.719 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 2 hour post bypass	2.352 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 0 time point	55.229 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 6 hour post bypass	26.524 pg/ml
Nesiritide	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 baseline	1.750 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 6 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 baseline	1.959 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 8 hour post bypass	0.599 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 time point 0	63.930 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 1 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 1 hour post bypass	57.412 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 24 hour post bypass	0.172 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 2 hour post bypass	43.523 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 8 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 4hour post bypass	37.967 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 baseline	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 6 hour post bypass	27.840 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 6 hour post bypass	575.429 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 8 hours post bypass	21.673 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 0 timepoint	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-6 24 hours post bypas	34.650 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 24 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 baseline	5.238 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 1 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8- 1 hour post bypass	20.873 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 1 hour post bypass	57.405 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8-2 hour post bypass	23.607 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 2 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 4 hour post bypass	17.627 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 baseline	0.969 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 6 hour post bypass	19.880 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 4 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 8hour post bypass	16.282 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 8 hour post bypass	449.376 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL8 24 hour post bypass	14.600 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL_3 6 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL10 baseline	9.130 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 0 timepoint	3.093 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL10 0 time point	2206.177 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-3 8 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 1 hour post bypass	2074.906 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 0 time point	150.567 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL10 2 hour post bypass	844.352 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL_3 24 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 4 hour post bypass	230.101 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 6 hour post bypass	95.925 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 1 hour post bypass	3.981 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 baseline	0.166 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 8 hour post bypass	84.070 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 24 hour post bypass	241.467 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL 10 24 hour post bypass	34.862 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 0 timepoint	0.359 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha baseline	5.402 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 2 hour post bypass	4.641 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 0 time point	7.795 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 1 hour post bypass	0.414 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 1hour post bypass	13.310 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 2 hour post bypass	40.900 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 2 hour post bypass	11.729 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 4 hour post bypass	4.423 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 4 hour post bypass	9.291 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 8 hour post bypass	18.522 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 6 hour post bypass	7.953 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha baseline	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 8 hour post bypass	5.212 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 24 hour post bypass	16.082 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF alpha 24 hour post bypass	4.525 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 6 hour post bypass	4.395 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 2 hour post bypass	0.406 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta baseline	0.056 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF baseline	43.984 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 0 time point	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 2 hour post bypass	1.185 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 1 hour post bypass	253.527 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 1 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 8 hour post bypass	3.316 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 4 hour post bypass	0.074 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 2 hour post bypass	297.668 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 6 hour post bypass	0.219 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 0 time point	10.109 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 8 hour post bypass	0.058 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 4 hour post bypass	311.452 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	TNF beta 24 hour post bypass	0.404 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-15 24 hour post bypass	4.996 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF baseline	44.971 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 6 hour post bypass	224.425 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 0 time point	35.773 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 4 hour post bypass	24.018 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 1 hour post bypass	48.928 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 8 hour post bypass	243.292 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 4 hour poswt bypass	30.529 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 baseline	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 6 hour post bypass	26.226 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	G-CSF 24 hour post bypass	199.849 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 8 hour post bypass	36.577 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL_4 2 hour post bypass	1.381 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 24 hour post bypass	64.660 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 1 hour post bypass	9.182 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 baseline	182.708 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 4 hour post bypass	0.958 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 0 timepoint	455.129 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 0 time point	0.117 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 1 hour post bypass	572.955 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 6 hour post bypass	0.746 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 2 hour post bypass	502.992 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta baseline	18.167 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 4 hour post bypass	325.249 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-4 8 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 6 hour post bypass	218.561 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 1 hour post bypass	0.130 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 8 hour post bypass	177.527 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL_4 24 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MCP-1 24 hour post bypass	215.708 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 2 hour post bypass	6.268 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF baseline	39.794 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 baseline	0.353 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 0 timepoint	21.983 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 2 hour post bypass	0.456 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 1 hour post bypass	19.395 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 0 timepoint	0.016 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 2 hour post bypass	23.662 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-8 0 time point	18.962 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 4 hour post bypass	19.141 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 1 hour post bypass	0.040 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 6 hour post bypass	22.655 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 4 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 8 post bypass	26.093 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 2 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	EGF 24 hour post bypass	17.768 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 4 hour post bypass	4.309 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha baseline	2.754 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 4 hour post bypass	0.016 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 0 time	31.597 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 6 hour post bypass	0.162 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 1 hour post bypass	21.069 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 6 hour post bypass	0.210 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 2 hour post bypass	17.803 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	VEGF 2 hour post bypass	41.695 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 4 hour post bypass	10.732 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 8 hour post bypass	0.431 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 6 hour post bypass	5.745 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL- 17 8 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 8 hour post bypass	5.376 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-5 24 hour post bypass	0.503 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1 alpha 24 hour post bypass	1.389 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 6 hour post bypass	0.870 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta baseline	0.394 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 baseline	2.987 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 0 time point	0.492 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-17 24 hour post bypass	0.0000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 1 hour post bypass	0.430 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 0 timepoint	4.311 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 2 hour post bypass	0.450 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 6 hour post bypass	20.263 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 4 hour post bypass	0.310 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 1 hour post bypass	1.822 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 6 hour post bypass	0.366 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 baseline	475.656 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 8 hour post bypass	0.462 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 2 hour post bypass	2.104 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1Beta 24 hour post bypass	0.170 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 8 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA baseline	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 4 hour post bypass	2.530 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 0 time point	53.721 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 0 time point	1790.035 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 1 hour post bypass	71.444 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 6 hour post bypass	2.317 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 2 hour post bypass	237.080 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 0 timepoint	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 4 hour post bypass	152.630 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 8 hour post bypass	2.354 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 6 hour post bypass	75.226 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 1 hour post bypass	2298.747 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 8 hour post bypass	27.442 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-7 24 hour post bypass	4.097 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-1RA 24 hour post bypass	15.765 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 alpha 24 hour post bypass	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 baseline	0.068 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 baseline	0.000 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 0 timepoint	0.401 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 2 hour post bypass	1365.169 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 1 hour post bypass	0.059 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	MIP-1 beta 0 time point	47.617 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 2 hour post bypass	1.110 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-13 4 hour post bypass	0.287 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 4 hour post bypass	0.870 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IP-10 4 hour post bypass	855.843 pg/ml
Placebo	The Examine and Measure Cytokines to Measure Kidney Injury	IL-2 6 hour post bypass	0.923 pg/ml

Primary

The Examine and Measure Endothelin-1 to Measure Kidney Injury

Serial measurements of Endothelin-1 levels were measured to determine if natriuretic peptides exert their renal protective effects by preserving renal afferent arteriole flow by antagonizing the vasoconstrictive effects of Endothelin-1.

Time frame: 30 days from the start of infusion

Population: randomized as outlined in the protocol

Arm	Measure	Group	Value (MEDIAN)
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	baseline prior to infusion	1.778 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	time point 0	2.096 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	1 hour post bypass	1.670 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	2 hour post bypass	2.173 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	6 hour post bypass	2.627 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	8 hour post bypass	2.744 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	24 hour post bypass	1.900 pg/ml
Nesiritide	The Examine and Measure Endothelin-1 to Measure Kidney Injury	4 hour post bypass	2.395 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	4 hour post bypass	3.116 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	baseline prior to infusion	1.239 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	8 hour post bypass	3.143 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	time point 0	2.283 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	6 hour post bypass	3.485 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	1 hour post bypass	2.068 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	24 hour post bypass	2.269 pg/ml
Placebo	The Examine and Measure Endothelin-1 to Measure Kidney Injury	2 hour post bypass	2.201 pg/ml

Primary

The Examine and Measure Urinary NGAL to Measure Kidney Injury

Urinary NGAL,a biomarker for kidney injury was measured.

Time frame: A change in urinary NGAL 2 hours after bypass was stopped and 5 minutes after the start of spontaneous circulation was resumed.

Population: randomized as outlined in protocol

Arm	Measure	Value (MEDIAN)
Nesiritide	The Examine and Measure Urinary NGAL to Measure Kidney Injury	124.566 ng/ml
Placebo	The Examine and Measure Urinary NGAL to Measure Kidney Injury	33.766 ng/ml

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026

Does Nesiritide Provide Renal Protection

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Outcome results

Measure Neutrophils to Measure Kidney Injury

The Examine and Measure Cytokines to Measure Kidney Injury

The Examine and Measure Endothelin-1 to Measure Kidney Injury

The Examine and Measure Urinary NGAL to Measure Kidney Injury