Cardiovascular Disease, Insulin Resistance, Obesity, Type 2 Diabetes
Conditions
Keywords
Advanced glycation endproducts, Satiety, Obesity, Inflammation, Insulin resistance
Brief summary
The purpose of the meal study is to investigate acute effects on satiety and inflammation of advanced glycation endproducts (AGE) in healthy overweight subjects. The AGE content of the meal is affected by food preparation methods: frying/grilling versus boiling/steaming.
Interventions
Test meals with different AGE content made by different cooking methods
Sponsors
The Ministry of Science, Technology and Innovation, Denmark
University of Copenhagen
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy males and females between 20 and 50 years old * BMI 25-40 * Waist circumference above 88 cm for women and above 102 cm for men
Exclusion criteria
* Exercise training above 8 hour/week * Smoking * Pregnant or breast-feeding women * Post-menstrual women * Regular use of medicine (except contraceptive pills) * Previous gastric bypass surgery * Donation of blood within the last 3 months * Involvement in other clinical trials * Allergic to paraaminobenzoic acid
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Appetite sensation | Every 30 min up to 5 hours after test meal | Appetite sensation measured by visual analogue scales |
| Plasma ghrelin | Continuous measurement up to 5 hours after test meal | — |
| Plasma GLP-1 | Continuous measurements up to 5 hours after test meal | — |
| Plasma PYY | Continuous measurements up to 5 hours after test meal | — |
| Plasma insulin | Continuous measurements up to 5 hours after test meal | — |
| Plasma glucose | Continuous measurements up to 5 hours after test meal | — |
| Urine AGE | From 24 hour before to 48 hour after test meal | — |
Secondary
| Measure | Time frame |
|---|---|
| ICAM | Continuous measurements up to 5 hours after test meal |
| MIF | Continuous measurements up to 5 hours after test meal |
| Plasma triglycerides | Continuous measurements up to 5 hours after test meal |
| Urine isoprostanes | From 24 hour before to 24 hours after test meal |
| Plasma AGE | Continuous measurements up to 48 hours after test meal |
| Expression of genes related to AGE | Continuous measurements up to 24 hours after test meal |
| IL-1 beta | Continuous measurements up to 5 hours after test meal |
| IL-6 | Continuous measurements up to 5 hours after test meal |
| TNF-alpha | Continuous measurements up to 5 hours after test meal |
| CRP | Continuous measurements up to 5 hours after test meal |
| VCAM | Continuous measurements up to 5 hours after test meal |
Countries
Denmark
Outcome results
None listed