Childhood Germ Cell Tumor, Extragonadal Germ Cell Tumor, Ovarian Cancer, Testicular Germ Cell Tumor
Conditions
Keywords
recurrent childhood malignant germ cell tumor, recurrent extragonadal germ cell tumor, recurrent malignant testicular germ cell tumor, recurrent ovarian germ cell tumor
Brief summary
RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat cancer. PURPOSE: This research trial studies samples from younger patients with malignant germ cell tumor progression.
Detailed description
OBJECTIVES: * Explore inter-tumoral heterogeneity in DNA methylation by tumor histology. * Determine the genomic methylation pattern in the tumors. * Correlate methylation pattern with tumor histology and clinical characteristics. * Carry out exome capture and massively parallel sequencing on selected germ cell tumors (GCTs) and matched normal tissue. * Perform exome capture and Solexa sequencing on a selected set of GCTs. * Validate candidate mutations in an independent set of tumors. * Determine the expression profile of mRNAs, lincRNAs and microRNAs in the tumors using RNA Seq. OUTLINE: Archived blood and tumor tissue samples are analyzed for genomic methylation pattern, exome capture and sequencing, and candidate mutations by methylation-specific PCR techniques, single nucleotide polymorphism (SNP) arrays, and Solexa sequencing methods. Results are validated by using pyrosequencing assays and primer-extension assays. Methylation pattern is also associated with each patient's tumor histology and clinical data.
Interventions
GENETICDNA methylation analysis
GENETICRNA analysis
GENETICmutation analysis
GENETICnucleic acid sequencing
GENETICpolymerase chain reaction
GENETICpolymorphism analysis
OTHERlaboratory biomarker analysis
OTHERmedical chart review
Sponsors
National Cancer Institute (NCI)
Children's Oncology Group
Study design
Observational model
CASE_ONLY
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Tumor and blood specimens from patients registered on the Children Oncology Group (COG) Germ Cell Tumor Protocols, and from other study sites for non-COG patients, including Children's Medical Center, Dallas and the Dana-Farber Cancer Institute, Boston * Patients' clinical data PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Event-free survival | — |
| Genomic prognostic signatures associated with GCTS | — |
| Genetic variants that contribute to GCTS pathogenesis | — |
| Expression of various forms of RNA | — |
Outcome results
None listed