DNA Tests in Detecting Disseminated Disease in Tumor, Blood, and Bone Marrow Samples From Patients With Rhabdomyosarcoma

DNA Methylation-based Assays for Detecting Disease Spread in Rhabdomyosarcoma

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01419509

Enrollment

170

Registered

2011-08-18

Start date

2011-08-31

Completion date

2016-05-31

Last updated

2016-05-17

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Sarcoma

Keywords

alveolar childhood rhabdomyosarcoma, embryonal childhood rhabdomyosarcoma, embryonal-botryoid childhood rhabdomyosarcoma, mixed childhood rhabdomyosarcoma, pleomorphic childhood rhabdomyosarcoma, previously untreated childhood rhabdomyosarcoma, nonmetastatic childhood soft tissue sarcoma

Brief summary

RATIONALE: DNA analysis of tumor, blood, and bone marrow may help doctors predict if the disease has been disseminated. PURPOSE: This research trial studies DNA tests in detecting disseminated disease in tumor, blood, and bone marrow samples from patients with rhabdomyosarcoma.

Detailed description

OBJECTIVES: * To establish a panel of hypermethylation assays for minimal disseminated disease analysis in rhabdomyosarcoma (RMS). * To apply the hypermethylation assays to a series of bone marrow specimens from RMS patients. OUTLINE: Archived tumor, bone marrow, and peripheral blood samples are analyzed for DNA hypermethylation by PCR-based assays and microarray assays. Results are then compared with each patient's clinical and pathological characteristics, and outcomes. Patterns of locus hypermethylation in tumor and paired bone marrow samples are also compared.

Interventions

GENETICDNA methylation analysis

GENETICmicroarray analysis

GENETICpolymerase chain reaction

OTHERlaboratory biomarker analysis

Study design

Observational model

CASE_ONLY

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

No minimum to 18 Years

Healthy volunteers

Inclusion criteria

DISEASE CHARACTERISTICS: * Diagnosed with rhabdomyosarcoma (RMS) * Tumor, bone marrow, and peripheral blood samples from the Children's Oncology Group D9803 trial of intermediate-risk RMS PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified

Design outcomes

Primary

Measure	Time frame
Panel of hypermethylation assays for minimal disseminated RMS	—
Detection of RMS cells in bone marrow	—

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026