Healthy
Conditions
Keywords
beef, protein, synergy, intact beef constituents, protein constituent, Nutrient Synergy
Brief summary
This study will compare the effects of cooked, ground beef to those of beef protein isolate on blood and muscle proteins.
Detailed description
STUDY OBJECTIVES Primary: To compare the effects of consumption of a 3-ounce serving of beef to that of a 20g serving of BPI on skeletal muscle synthetic and breakdown rate in healthy elderly subjects. Secondary: To investigate the effect the study products on plasma levels of amino acids, insulin, and glucose in healthy elderly subjects.
Interventions
DIETARY_SUPPLEMENT3 ounces of cooked, 85% lean ground beef
Consumed once per subject.
DIETARY_SUPPLEMENT20 grams beef protein isolate
Subjects would drink 200 ml water mixed with 20 grams of beef protein isolate one time.
Sponsors
University of Arkansas
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
60 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Healthy male or female. * Age 60 years or older.
Exclusion criteria
* Any (history of) gastrointestinal disease that interferes with GI function * Diagnosed and active treatment of Diabetes Mellitus type I or II * History of congestive heart failure * Recent (6 months) hospitalization for heart disease treatment or management (e.g. PTCA, stent, surgery) * Myocardial infarction in the past year * Infection or fever in the 7 days prior to enrollment * Current use of corticosteroids, growth hormone, or testosterone * Adherence to a weight loss diet * Use of protein-containing or amino acid-containing nutritional supplements within one week of enrollment * Current alcohol (\>7 ETOH per week) or drug abuse * Platelet count (PLT) \< 100,000 * History of hypo- or hyper-coagulation disorders including use of a coumadin derivative * Currently taking anti-thrombotics and cannot stop for 7 days (i.e. medical indication) * Allergy to local anesthetic * Blood hemoglobin \< 9.0 g/dL * Investigator's uncertainty about the willingness or ability of the subject to comply with the protocol requirements
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| skeletal muscle synthetic and breakdown rate | day one | Using stable isotope infusion/ingestion methodology, we will measure plasma and skeletal muscle metabolic rate. Our primary end points will be muscle protein fractional synthesis rate (FSR) and whole body protein turnover rate. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| concentrations of plasma amino acids, insulin and glucose | day one | Secondary endpoints are concentrations of plasma amino acids, insulin and glucose. |
Countries
United States
Outcome results
None listed