Healthy
Conditions
Keywords
meat, LDL, MDA
Brief summary
The postprandial increase in MDA level following meat meal could cause modification to LDL particles in healthy volunteers. Co-consumption of food drived polyphenols with the meat will result in a significant decrease in LDL modification.
Interventions
OTHERfrozen meat cutlets meal
250 g of meat cutlets
OTHERmeat meals
250 g of meat meal for 4 days
Sponsors
Hadassah Medical Organization
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy adults, normal BMI
Exclusion criteria
* Metabolic disorders * Smokers * Drinkers * Taking nutritional supplement
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in plasma MDA levels following meat meal | Fasting before meal and 3, and 6 h postprandial, and fasting levels | After an overnight fast baseline (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The subjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 and 6 h after the meal was eaten. Serum was separated from whole blood by centrifugation (910 g, 15 min), and MDA was evaluated by HPLC in plasma samples |
| Changes in MDA concentration on LDL fraction following meat meals | Fasting, before meal was eaten, 3 h following meal, at day 4, 3 h following meal consumption, and day 5 following over nigth fast | Subject consumed the test meals for 4 days. Day 1, (0 h) fasting blood sample (10 ml) was collected in EDTA-treated tubes. The sbjects then ate a test meal within 30 min. Blood samples (10 ml) were drawn 3 h after the meal was eaten. Blood was collected at day 4, 3 h after the meal was eaten, at the morning of day 5.Plasma LDL was isolated using a single discontinuous density gradient ultracentrifugation. The protein concentration was determined based on the method of Bradford. MDA concentration was evaluated by HPLC. |
Countries
Israel
Outcome results
None listed