SPERM
Conditions
Brief summary
The aim of this study is evaluated the effect of dietetic supplementation with DHA on human sperm quality, antioxidant capacity and sperm DNA damage.
Interventions
DIETARY_SUPPLEMENTSunflower oil
1500 mg sunflower/day for 10 weeks
DIETARY_SUPPLEMENTDocosahexanoic acid
1500mg/day for 10 weeks
Sponsors
University of Barcelona
Instituto Valenciano de Infertilidad, IVI VALENCIA
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Healthy volunteers
No
Inclusion criteria
* Diagnosis of male factor.
Exclusion criteria
* Metabolic and genetic diseases. * Oncological patients. * Patients with anticoagulants treatment.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fatty acids measure. | 5 weeks | Fatty acid analysis in seminal plasma and spermatozoa were evaluated by gas chromatography.The percentage of each fatty acid class was expressed as percentage of total fatty acids. |
| Sperm morphology | 5 weeks | Percentage of sperm with normal morphology |
| Total capacity antioxidant | 5 weeks | Total antioxidant capacity (TAC)is a colorimetric assay that measure the combined antioxidant activities of all its non enzymatic constituents in seminal plasma. |
| Sperm DNA damage. | 5 weeks | DNA fragmentation analysis was evaluated by TUNEL test and measured by flow cytometry |
| sperm concentration | 5 weeks | million of spermatozoa per milimitre |
| Sperm motility | 5 weeks | Percentage of motile sperm. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Volume of ejaculate. | 5 weeks | Eyaculated milliliters. |
| Sperm viability | 5 weeks | Porcentage of sperm whitout membrane damage |
| Sperm in ejaculate | 5 weeks | Million of spermatozoa in eyaculate. |
Countries
Spain
Outcome results
None listed