Lymphoma
Conditions
Keywords
childhood anaplastic large cell lymphoma
Brief summary
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors identify and learn more about biomarkers related to cancer. it may also help doctors predict how patients will respond to treatment. PURPOSE: This research trial studies biomarker expression in tissue samples from young patients with anaplastic large cell lymphoma.
Detailed description
OBJECTIVES: * To determine the expression of immunoregulatory proteins including CD4, FOXP3 and CD276 in anaplastic large cell lymphoma (ALCL). * To correlate the expression of these proteins with the levels of anaplastic lymphoma kinase (ALK) autoantibodies in patient of ANHL0131. OUTLINE: Archived tissue microarray samples are analyzed by mass spectrometry and validated by Western blotting, flow cytometry, and IHC.
Interventions
GENETICmicroarray analysis
GENETICprotein expression analysis
GENETICwestern blotting
OTHERflow cytometry
OTHERimmunohistochemistry staining method
OTHERlaboratory biomarker analysis
OTHERmass spectrometry
Sponsors
National Cancer Institute (NCI)
Children's Oncology Group
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 20 Years
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Diagnosis of anaplastic large cell lymphoma * Tissue microarray samples available from the Cooperative Human Tissue Network (CHTN) PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Expression of immunoregulatory proteins including CD4, FOXP3 and CD276 in ALCL | — |
| Correlation of CD4, FOXP3, and CD276 with ALK autoantibody response in patients enrolled in ANHL0131 | — |
Outcome results
None listed