Sarcoma
Conditions
Keywords
alveolar childhood rhabdomyosarcoma, embryonal childhood rhabdomyosarcoma, previously treated childhood rhabdomyosarcoma, previously untreated childhood rhabdomyosarcoma, recurrent childhood rhabdomyosarcoma
Brief summary
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in tumor tissue samples from patients with rhabdomyosarcoma.
Detailed description
OBJECTIVES: * To determine and quantify the degree of surface insulin-like growth factor I receptor (IGF-IR) expression in rhabdomyosarcoma (RMS) tumor tissue. * To assess the feasibility and validity of a mass spectrometry assay for IGF-IR expression on paraffin-embedded tumor tissue. OUTLINE: Paraffin-embedded tumor tissue slides are analyzed for insulin-like growth factor I receptor (IGF-1R) expression by mass spectrometry and correlated with the results obtained by IHC assay.
Interventions
GENETICgene expression analysis
OTHERimmunohistochemistry staining method
OTHERlaboratory biomarker analysis
OTHERmass spectrometry
Sponsors
National Cancer Institute (NCI)
Children's Oncology Group
Study design
Observational model
CASE_ONLY
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
DISEASE CHARACTERISTICS: * Diagnosis of rhabdomyosarcoma * Alveolar or embryonal rhabdomyosarcoma * Available samples from the Cooperative Human Tissue Network (CHTN) bank PATIENT CHARACTERISTICS: * Not specified PRIOR CONCURRENT THERAPY: * Not specified
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Degree of surface IGF-IR expression in RMS tumor tissue | — |
| Feasibility and validity of a mass spectrometry assay for IGF-IR expression on paraffin-embedded tumor tissue | — |
Outcome results
None listed