Cardiovascular Risk Factors
Conditions
Keywords
Endothelial function, Non-esterified fatty acids, Insulin sensitivity
Brief summary
Experimental elevation of non-esterified fatty acids (NEFA) impairs endothelial function and insulin sensitivity but the impact of NEFA composition is unknown. The objective was to test the effect of acute elevation of NEFA enriched with either saturated fatty acids (SFA) or SFA with long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) on postprandial vascular function measured via flow-mediated dilatation (FMD), laser Doppler iontophoresis (LDI) and digital volume pulse (DVP), followed by a hyperinsulinaemic-euglycaemic clamp as a measure of whole body insulin sensitivity.
Detailed description
To investigate potential diet-gene interactions, potential subjects (n=370) were prospectively genotyped for the eNOS Glu298Asp polymorphism, of which 35 were Asp298 and 150 were Glu298 homozygotes. Three subjects in the Asp298 group were unable to participate, two were unsuitable according to selection criteria and one subject subsequently withdrew from the study. Subjects homozygous for Asp298 (n=29) and Glu298 (n=30) were therefore selected, balanced for gender, age and BMI.
Interventions
DIETARY_SUPPLEMENTAbsence or presence of fish oil
Oral dose of saturated fat with or without fish oil and a heparin infusion
Sponsors
Biotechnology and Biological Sciences Research Council
Unilever R&D
Foundation for Research Science and Technology (New Zealand)
University of Reading
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Either homozygous for eNOS Glu298 (wildtype)or eNOS Asp298 (variant)
Exclusion criteria
* Smokers * Raised fasting blood lipids * Taking excessive fish oil supplements (\>1g EPA/DHA per day) * Taking medication known to influence blood lipids, blood pressure or blood clotting
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Flow-mediated dilatation | Change from baseline to 240 min |
Secondary
| Measure | Time frame |
|---|---|
| Laser Doppler iontophoresis | Change from baseline to 240 min |
| Insulin sensitivity | 390 min |
| Digital volume pulse | Change from baseline to 240 min |
| Circulating endothelial function markers | Change from baseline to 240 min |
| Circulating endothelial markers | Change from 240 min to 390 min |
| NEFA composition | Change from baseline to 240 min |
Countries
United Kingdom
Outcome results
None listed