Candidiasis
Conditions
Keywords
ICU
Brief summary
Our primary objective is to evaluate the relevance of the early determination of Candida infection status among non-neutropenic patients hospitalized over 48 hours in ICUs.
Interventions
BIOLOGICALTests for candidiasis
Tests include (1) anti-candida antibody ELISA kit; (2) candida antigen ELISA kit; (3) detection of circulating Candida DNA via SYBR Breen qPCR Master mix; (4) colonization index based on mycological examination of five out of the following: urine, oral swab, bronchial aspirate, anal swab, swab of a central venous line, gastric liquid.
Sponsors
Centre Hospitalier Universitaire de Nīmes
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Polynuclear neutrophils \> 500/mm\^3 * Hospitalized for \> 48 hours in participating ICUs
Exclusion criteria
* Patients discharged from ICU in \< 48h * Patient diagnosed with invasive candidosis before entry to ICU * Patient is taking an anti-fungal treatment * polynuclear neutrophils \< 500/mm\^3 * patient transferred to another ICU * patient included in the Abmidex protocole
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of positive Candidiasis tests | 7 days | Number of tests (score of 0 to 4) positive for Candida. Tests include (1) anti-candida antibody ELISA kit; (2) candida antigen ELISA kit; (3) detection of ciruclating Candida DNA via SYBR Breen qPCR Master mix; (4) colonization index based on mycological examination of five out of the following: urine, oral swab, bronchial aspirate, anal swab, swab of a central venous line, gastric liquid. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Number of positive Candidiasis tests | 2 days | Number of tests (score of 0 to 4) positive for Candida. Tests include (1) anti-candida antibody ELISA kit; (2) candida antigen ELISA kit; (3) detection of ciruclating Candida DNA via SYBR Breen qPCR Master mix; (4) colonization index based on mycological examination of five out of the following: urine, oral swab, bronchial aspirate, anal swab, swab of a central venous line, gastric liquid. |
| Patient leaves ICU unit, yes/no | 1 month | — |
| Patient passes away in ICU unit, yes/no | 1 month | — |
| Patient has been prescribed an antifungal treatment in the past month, yes/no | 1 month | — |
Countries
France
Outcome results
None listed