Allergy
Conditions
Keywords
1Allergy, 2 Neuromuscular blockers
Brief summary
To assess diagnostic accuracy of flow cytometry applied to the diagnosis of allergy to neuro-muscular blockers and to the determination of the neuro-muscular blocker (NMB) which may be used for an ulterior anaesthesia in case of allergy to one given NMB.
Detailed description
The flow cytometric protocol used for this study, was based on basophil selection by an anti- CCR3 and the analysis of basophil activation by the respective up and down regulation of the CD63 and IgE markers as described previously. Basophil activation was expressed in percentage CD63 expression on activated basophils and in activation index involving IgE and CD63 markers and calculated by a specific algorithm developed by our group.. Our aim is to test a sufficient number of patients in order to be able to establish the ROC curves related to the allergen type tested and validate the use of an allergen mixture (NMB mix containing the 7 usual NMB at the optimal activation concentrations). Patients will be included during the post-anaesthetic consultation on the basis of the diagnosis of an IgE dependant per-anaesthetic reaction. The allegro-anaesthetist diagnostical habits won't be changed.
Interventions
OTHERBlood simple
Single Blood simple at Day 1
Sponsors
University Hospital, Limoges
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* patients aged at least 18 years, * patients having experienced an IgE dependant per-anaesthetic reaction * affiliated to a social security
Exclusion criteria
* patients having taken anti-histamine drugs and corticoids during the last 8 days * patients submitted to a juridical protection
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Allergy diagnosis assessed by cytometry flow data | At Day 1 |
Countries
France
Outcome results
None listed