Role of the Gut Sweet Taste Receptor in the Secretion of Glucagon-like Peptide-1 and Peptide YY

The Role of Sweet Taste Receptor Blockade on Intraduodenal Glucose- and Liquid Meal-stimulated Secretion of Satiation Peptides in Healthy Humans

Status

Completed

Phases

Phase 1

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT01304823

Enrollment

Registered

2011-02-25

Start date

2010-06-30

Completion date

2011-01-31

Last updated

2015-07-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Taste Disorder, Primary, Sweet

Keywords

T1R2/T1R3, gastric emptying, gut, lactisole, nutrient sensing

Brief summary

The purpose of this study is to determine the functional significance of sweet taste receptors in the secretion of GI satiation peptides by using a specific sweet taste receptor antagonist to block sweet taste perception in the gastrointestinal tract.

Detailed description

There is strong evidence that taste signaling mechanisms identified in the oral epithelium also operate in the gut. It is suggested that open-type enteroendocrine cells directly sense nutrient via alpha-gustducin coupled taste receptors to modulate the secretion of glucagon like peptide-1 (GLP-1) and peptide YY (PYY). Several nutrient responsive G-protein coupled receptors have been identified in the human gut, including the sweet taste responsive T1R2/T1R3 heterodimer, the amino acid/umami responsive T1R1/T1R3 as well as GPR120 for unsaturated long-chain free fatty acids.The functional significance of sweet taste receptors in the secretion of GLP-1 and PYY will be determined by intraduodenal perfusion of glucose (I) or a mixed liquid meal (II) with or without lactisole (450 ppm) in a double blind, 5-way crossover trial including 10 healthy subjects.

Interventions

DIETARY_SUPPLEMENTlactisole

flavoring agent/sweet taste antagonist

OTHERmixed liquid meal

Ensure Plus

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)

Eligibility

Sex/Gender

MALE

Age

18 Years to 40 Years

Healthy volunteers

Yes

Inclusion criteria

* Healthy subjects * BMI of 19.0-24.5 * Age 18-40 * stable body weight for at least 3 month

Exclusion criteria

* smoking * substance abuse * regular intake of medication * medical or psychiatric illness * gastrointestinal disorders or food allergies

Design outcomes

Primary

Measure	Time frame	Description
Gastrointestinal peptide secretion	4 hours blood sampling	Assessment of venous GLP-1 and PYY release to meal stimulation with and without sweet receptor blockade

Secondary

Measure	Time frame	Description
Appetite perceptions during 4 hours using VAS	4 hours	Assessment of the following appetite perception markers: feelings of hunger, feelings of fullness, feelings of satiety using validated visual analogue scales

Countries

Switzerland

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026