Proteinic Synthesis, Proteolytic System, Transduction Pathway
Conditions
Brief summary
Gut protein metabolism that could be influenced by protein supply is involved in the regulation of several physiological functions such as gut barrier function and represents a major contribution in the whole-body protein turnover. Intracellular pathways implicated in the transduction of amino acids effects remained unknown in the human gut. Thus, the aim of the present project is to evaluate and to understand the effects of amino acids (glutamine, leucine) on gut protein metabolism (synthesis and degradation) in young healthy humans.
Interventions
None listed
Sponsors
University Hospital, Rouen
Study design
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* volunteers with seronegativity for HIV1 and HIV2 and HCV and HVB * good nutritional state with physical indication mass included between 18,5 and 25kg/m2 * non-smoking volunteers * volunteers without digestive, allergic or haemorrhagic history * volunteers without progressive disease * digestive endoscopy tolerance * age \>=18 and \<= 50years * consent obtained from volunteers
Exclusion criteria
* smoking volunteers * volunteers with digestive, or allergic history * volunteers with haemorrhagic sign * pregnancy * progressive digestive disease * significant progressive disease * volunteers with therapeutic treatment (aspirin) * digestive endoscopy intolerance * volunteers with neurological disorder or with delicate psychological state
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| assessment of intestinal protein synthesis stimulation by Leucine or glutamine in duodenal biopsy | 18 months |
Secondary
| Measure | Time frame |
|---|---|
| Assessment of involved signaling pathways | 18 months |
| Assessment of intestinal proteolysis | 18 months |
| Assessment of protein synthesis rate in intestinal mitochondria | 18 months |
Countries
France
Outcome results
None listed