Obesity
Conditions
Keywords
Nutrition, Lipid, Digestion, Endotoxemia, Inflammation, Metabolism, Stable Isotope, Obesity
Brief summary
In this study, the investigators are interested in investigating how the emulsification state and amount of fat in a meal can modify the kinetics of postprandial lipemia and inflammatory outcomes (including endotoxemia) in obese vs lean subjects. 10 lean and 10 obese volunteers will come 3 times (\>3 weeks apart) at the investigation center to receive a standard breakfast containing milk fat (10 g non emulsified, or 40 g non emulsified, or 40 g finely emulsified). 13C triglyceride stable isotopes will allow to follow the metabolic fate of fatty acids and calculate lipid oxidation by breath test. Blood sampling throughout digestion will allow to measure metabolic, lipid and inflammation parameters. Stool will be analysed to determine lipid losses in faeces and to phenotype microbiota.
Interventions
OTHERAll subjects receive three sequences of a standard breakfast
All subjects receive three sequences of a standard breakfast containing : * 10 g non emulsified * or 40 g non emulsified * or 40 g finely emulsified of milk fat with stable isotope (13C triglyceride). The administration order is determined by randomized allocation. The wash-out period is three weeks. There are not diet or exercise interventions. Only, one week before and three days after, the subjects consume neither naturally enriched in 13C foods or pre/probiotics. In addition, three days before and three days after each test, the subjects receive dietary instructions
Sponsors
Hospices Civils de Lyon
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* No smokers * BMI 18 to 35 kg/m2 * Moderate physical activity * Safety subject during medical consultation
Exclusion criteria
* Medical history which may affect lipid metabolism and gut microflora (renal -cardiovascular -hepatic- endocrine-inflammatory diseases) * Drug use that could affect lipid metabolism and gut microflora (steroids, antilipemic agent, anorectic, antibiotic) * Inflammatory or infectious disease in the last three month * C Reactive Protein \> 20 * Prebiotic or probiotic high consumers (several times a day) * Eating disorder * Claustrophobic subjects
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Time of appearance of plasma triglyceride peak after ingestion of emulsified vs unemulsified fat during breakfast | four hours |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Kinectics of: Lipid oxidation plasma metabolite concentrations Endotoxemia and plasma markers of inflammation 13 C-fatty acid appearance in plasma | one day | Subgroups analysis on subjects BMI amount of ingested fat and emulsified or unemulsified state of ingested fat |
Countries
France
Contacts
Primary ContactMartine LAVILLE, PU-PU
Outcome results
None listed