Parodontitis Aggressive, Parodontitis Chronic
Conditions
Brief summary
The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.
Interventions
PROCEDUREBiopsy
dental extraction and gingival epithelial biopsy
PROCEDUREbiopsy
dental extraction and gingival epithelial biopsy
Sponsors
Centre Hospitalier Universitaire de Nice
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
12 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Men exclusively * 12 years minimum * 2 patients affected by chronic periodontitis * 2 patients affected by aggressive periodontitis * 2 healthy patients (orthodontics extraction) * Subject who have read and understood the information note relative under investigation and who have signed the informed consent * Subject whose legal representative signed the parental consent (if minor patient)
Exclusion criteria
* Patient with contre-indication to dental extraction * Patient witn serious blood disease * Patient who exhibits systemic condition incompatible with the realization of the study * Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants * Patient who presents za buccal carcinoma treated with radiotherapy * Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy * Patient who presents a linguistic or psychic incapacity to understand information * Female patient
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Presence/absence of EBV in the epithelial cells of periodontal pockets (by Immunofluorescence and in situ Hybridization | at time = 0 (baseline) |
Secondary
| Measure | Time frame |
|---|---|
| Presence/ absence of latency and/or lytic proteins (by immunofluorescence and in situ hybridization | at time = 0 (baseline) |
| - Epithelial tissue infected (by Immunofluorescence and In situ Hybridization) : detection of oral gingival epithelium, sulcular epithelium, junctional epithelium. | at time = 0 (baseline) |
Countries
France
Outcome results
None listed