Aggressive Periodontitis, Chronic Periodontitis, Presence / Absence of EBV
Conditions
Brief summary
The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis.
Interventions
PROCEDUREdental root planing
The objective of root planing, otherwise known as conventional periodontal therapy, non-surgical periodontal therapy, or dental prophylaxis is to remove or eliminate the etiologic agents which cause inflammation: dental plaque, its products and calculus,\[1\] thus helping to establish a periodontium that is free of disease.\[2\]
Sponsors
Centre Hospitalier Universitaire de Nice
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
HEALTH_SERVICES_RESEARCH
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* 10 patients affected by chronic periodontitis * 10 patients affected by aggressive periodontitis * 10 healthy patients (orthodontics extraction) * Subject who have read and understood the information note relative under investigation and who have signed the informed consent * major patient
Exclusion criteria
* Patient with no contre-indication to periodontal treatment * Patient with serious blood disease * Patient who exhibits systemic condition incompatible with realization of the study * Patient treated with oral retinoids, bisphosphonates, oral anticoagulants and anticonvulsivants * Patient who presents a buccal carcinoma treated with radiotherapy * Patient who have received during the past 6 months: anti-inflammatory drugs, anti-cancerous or immunosuppressive chemiotherapy * Patient who presents a linguistic or psychic incapacity to understand information
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Percentage of gingival epithelial cells infected by EBV (by immunofluorescence and In situ Hybridization) | at time=0 |
Secondary
| Measure | Time frame |
|---|---|
| Epithelial tissue infected by EBV (by immunofluorescence and in situ hybridization) | at time = 0 |
| Presence / absence of latency and / or lytic proteins (by immunofluorescence and in situ hybridization) | at time = 0 |
Countries
France
Outcome results
None listed