Aging, Calorie Restriction
Conditions
Keywords
fasting, starvation, calorie restriction, fMRI
Brief summary
Calorie restriction extends life span and prevents aging-related diseases in several species. Odorants from live yeast restrain the beneficial effects of calorie restriction in Drosophila Melanogaster. The investigators hypothesize that visual and odorous food stimuli impact the neuroendocrine and metabolic response to starvation in healthy humans. In this randomized cross-over intervention study 12 healthy, young men will fast twice for 60-hours in the presence or absence of food-related visual and odorous stimuli. At baseline and on the last morning of each intervention an oral glucose tolerance test (OGTT) will be performed. During the OGTT blood is sampled and hypothalamic neuronal activity is measured by functional MRI.
Interventions
OTHERStimuli of food smell and vision
60 hours of starvation (fasting) in the presence or absence of visual and odorous food cues
OTHERNo stimuli of food smell and vision
60 hours of starvation (fasting) in the presence or absence of visual and odorous food cues
Sponsors
Center for Medical Systems Biology
Roba Metals BV, IJsselstein
Leiden University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Caucasian male volunteers * Age \> 18 years and \< 70 years * Body mass index (BMI) \> 20 kg/m2 and \< 25 kg/m2 * Fasting serum glucose (FSG) \< 6.1 mmol/L
Exclusion criteria
* Any significant chronic of hereditary disease (including renal, hepatic or endocrine disease) * Any significant abnormal laboratory results found during the medical screening procedure * Any use of medication (except for NSAID) * Anosmia or any other (acquired) loss of olfaction (ea. rhinitis) * Recent weight changes or attempts to loose weight (\> 3 kg weight gain or loss, within the last 3 months) * Difficulties to insert an intravenous catheter * Smoking * MRI contraindications * Recent blood donation (within the last 3 months) * Recent participation in other research projects (within the last 3 months), participation in 2 or more projects in one year
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Endocrine and metabolic outcomes | 4 weeks | The effects of odors and / or visual stimuli of food in subjects after 60h of starvation on: endocrine parameters, metabolic parameters, anthropometric parameters |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| functional Magnetic Resonance Imaging | 4 weeks | The effects of odors and / or visual stimuli of food in subjects after 60h of starvation on hypothalamic neuronal activity |
Outcome results
None listed