Oligospermia
Conditions
Keywords
methylation, micro array, locus H19, male infertility
Brief summary
This study will analyse the sperm global methylation status of 62 infertile men before assisted reproductive techniques. Some of these patients (20%) present hypomethylation of H19 locus. A global methylation analysis may reveal others imprinting defects.
Detailed description
An increase of the abnormalities of the imprint was brought back at the child's stemming from assisted reproductive techniques. Now abnormalities of methylation could be implied in defects of spermatogenesis and certain abnormalities of development of the male germ cells could be due to modifications abnormal epigenetics. The objective of this research is to determine the frequency of arisen the abnormalities of methylation at the level of the locus H19 in the sperm cells of barren men presenting an unexplained oligozoospermia and to determine if these changes are a reflection of abnormalities of the profiles of methylation of the whole genome. The patients will realize a taking of sperm having signed the consent.
Interventions
OTHERmethylation analyses on spermatozoa from infertile men
microarray analysis(www.EPIGENOMICS.com)
Sponsors
Assistance Publique - Hôpitaux de Paris
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* Men from 18 to 45 years old, presenting an idiopathic oligozoospermia lower than 10 million sperm cells / ml and include in a program of medically assisted conception * Patients with social security * Patients having signed the informed consent
Exclusion criteria
* Infertility with a neoplastic origin: patients subjected to a treatment potentially sterilizing (chemotherapy or radiotherapy). * Infertility with an infectious origin * Infertility with a traumatic origin * Infertility bound to a chromosomal abnormality or a microdeletion of Y * Histories of cryptorchidism, of varicocele
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Bring to light methylation abnormalities of the locus H19 in men's mature sperm cells presenting an unexplained oligozoospermia | 1 day |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Determine if these methylation abnormalities of the locus H19 reflect changes in the profile of global methylation of the spermatic DNA | 1 day | — |
| Estimate the association between these modifications and the nuclear quality of the sperm cell | 1 day | by TUNEL analysis |
| Estimate the association between these modifications and the rates of success with In VITRO fertilization | 1 day | — |
Countries
France
Outcome results
None listed