Skin Prick Test
Conditions
Keywords
allergy, skin prick test, mite sensitivity
Brief summary
The purpose of this study is to compare the results skin prick tests using simple blood lancet (Vitrex®) with DuoTips among mite-sensitized subjects.
Detailed description
We intend to compare results of skin prick tests by using simple blood lancet(Vitrex®) and DuoTips in terms of wheal size, variability, transfer of allergen and pain level, among mite-sensitive subjects.
Interventions
DEVICESimple blood lancet
Skin prick testing with blood lancet and DuoTip is performed on the back of mite-sensitized person. Allergen(Der p)is placed in 1st and 2nd row whereas histamine is placed in 3rd row. Allergen and normal saline are placed alternately in 4th row. Block randomization were allocated subjects into 2 groups; Group1:blood lancet is performed in 1st row and DuoTip in 2nd row. Group2:DuoTip is performed in 1st row and blood lancet in 2nd row. Blood lancet is applied in 3rd and 4th row in all subjects. A technician performed device applications and another technician who is blinded to device performed all readings. Pain was assessed on a scale from 0 to 10 with 0 being no pain and 10 being the worst imaginable pain
Sponsors
Siriraj Hospital
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
12 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Who have positive skin prick testing to Der p 10,000 AU/ml (wheal size 3 mm at least) * Age 12-60 yrs * Written informed consent was obtained from all subjects and their parents
Exclusion criteria
* Who have positive skin prick testing to Der p 10,000 AU/ml above 15 mm. * Who have dermographism * Who have severe disease eg. severe respiratory disease or severe cardiovascular disease * Who have severe eczema * Who receive antihistamine, topical steroid within 7 days or ketotifen within 14 days or systemic steroid within 1 month * Pregnant women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Wheal and flare reaction to Der p results of skin prick test by simple blood lancet (Vitrex®) | 15 minutes | To determine 1. Wheal and flare diameter to Der p performed by blood lancet(Vitrex®)compared with DuoTip in mite-sensitized person 2. Intradevice variability or coefficients of variation of blood lancet compared wtih DuoTip |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Determine transfer of allergen by simple blood lancet and Duotips. | 15 minutes | To determine 1. Transfer of allergen(Der p)performed by simple blood lancet(Vitrex®) 2. Pain assessment by using visual analog scale 3. Wheal and flare diameter to histamine performed by simple blood lancet(Vitrex®) |
Countries
Thailand
Outcome results
None listed