Aging
Conditions
Keywords
Oxidative Stress, Free radicals, Aging
Brief summary
The purpose of this study is to understand why and how oxidative stress negatively impacts mobility in the elderly, and to determine whether antioxidant supplements can increase vascular health and mobility.
Detailed description
With the premise that U.S. Veteran demographics reveal an aging population with significant mobility limitation and oxidative stress is tightly linked to both of these characteristics, a series of skeletal muscle/vascular studies are proposed. Specifically, in a series of recent studies the investigators' group has documented positive vascular consequences of antioxidant supplementation during exercise in older subjects that negatively impacted young people. Further provocative findings revealed that following exercise training the older subjects were now also negatively impacted by the antioxidant supplementation. These findings have implications for the understanding of the complex balance between the positive effects of exercise-based rehabilitation, exercise induced oxidative stress, aging, frailty, and subsequent mobility limitation. Therefore four specific aims are proposed that will answer the questions of where (I) oxidative stress is most prevalent in the elderly, why (II) oxidative stress occurs in the elderly, what (III) are the acute consequences of oxidative stress in the elderly, and finally how (IV) can exercise-induced oxidative stress as the result of exercise training in elderly be appropriately managed with exogenous antioxidant therapy to promote compliance and the positive outcome of increased mobility.
Interventions
DIETARY_SUPPLEMENTVitamins C, E, and alpha lipoic acid
Oral antioxidant cocktail or placebo to be consumed daily
Sponsors
University of Utah
VA Office of Research and Development
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
Participants will consume either a daily oral antioxidant cocktail or a placebo. The oral antioxidant cocktail consists of vitamin C, vitamin E, and alpha lipoic acid.
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women aged either between 18 - 30 years (young group) and \>70 years (old group) * Free of overt disease. Subjects will be non-obese (BMI \< 30) and have plasma glucose concentrations \< 7.0 mmol/L under fasting conditions and \< 11.1 mmol/L at 120 minutes of an oral glucose tolerance test (OGTT) * Subjects will be sedentary, defined here as no regular physical activity for at least the prior 2 years * Candidates must have no orthopedic limitations that would prohibit them from performing cycle or knee-extensor exercise * All women will be postmenopausal (either natural or surgical) defined as a cessation of menses for at least 2 years, and in women without a uterus, follicle stimulating hormone (FSH) \> 40 IU/L
Exclusion criteria
* Candidates demonstrating dyslipidemia based on the National Cholesterol Education Program Guidelines of plasma total cholesterol \> 240 mg/dl with LDL-cholesterol \> 160 mg/dl will be excluded from participation * Candidates who smoke or are taking medications (blood pressure medications, statins, antioxidants etc.) that could influence the results of the study will not be eligible * In terms of diet, subjects will be asked to maintain a dietary record for one week prior to the pre-screening and if this reveals a diet that differs substantially from the typical average diet in both the young and old, the subject will not be eligible * Women currently taking hormone replacement therapy (HRT) in the preceding year will be excluded from the proposed studies
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Vascular Function (i.e. % Flow Mediated Vasodilation) | 8 weeks | The effect on flow mediated vasodilation is measured using Doppler ultrasound. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Exercise-induced Oxidative Stress (i.e. Free Radical Concentrations and Levels of Lipid Peroxidation) | 8 weeks | Free radicals and levels of lipid peroxidation were measured using electron paramagnetic spectroscopy and ELISA assays. A minimum value is 0 and a maximum value is 10. A higher value is a worse outcome. |
| Vascular Function Following the Antioxidant Intervention | 8 weeks | The degree of vasodilation following the antioxidant intervention measured by Doppler ultrasound. |
Participant flow
Participants by arm
| Arm | Count |
|---|---|
| Arm 1 Young healthy men and women aged 18-30
Vitamins C, E, and alpha lipoic acid: Oral antioxidant cocktail or placebo to be consumed daily | 42 |
| Arm 2 Older healthy men and women aged \>70.
Vitamins C, E, and alpha lipoic acid: Oral antioxidant cocktail or placebo to be consumed daily | 45 |
| Total | 87 |
Baseline characteristics
| Characteristic | Arm 2 | Total | Arm 1 |
|---|---|---|---|
| Age, Categorical <=18 years | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical >=65 years | 45 Participants | 45 Participants | 0 Participants |
| Age, Categorical Between 18 and 65 years | 0 Participants | 42 Participants | 42 Participants |
| Race and Ethnicity Not Collected | — | 0 Participants | — |
| Region of Enrollment United States | 45 Participants | 87 Participants | 42 Participants |
| Sex: Female, Male Female | 17 Participants | 38 Participants | 21 Participants |
| Sex: Female, Male Male | 28 Participants | 49 Participants | 21 Participants |
Adverse events
| Event type | EG000 affected / at risk | EG001 affected / at risk |
|---|---|---|
| deaths Total, all-cause mortality | 0 / 42 | 0 / 45 |
| other Total, other adverse events | 0 / 42 | 0 / 45 |
| serious Total, serious adverse events | 0 / 42 | 0 / 45 |
Outcome results
Primary
Vascular Function (i.e. % Flow Mediated Vasodilation)
The effect on flow mediated vasodilation is measured using Doppler ultrasound.
Time frame: 8 weeks
| Arm | Measure | Value (MEAN) | Dispersion |
|---|---|---|---|
| Arm 1 | Vascular Function (i.e. % Flow Mediated Vasodilation) | 7.4 percentage of vasodilation | Standard Error 0.6 |
| Arm 2 | Vascular Function (i.e. % Flow Mediated Vasodilation) | 5.2 percentage of vasodilation | Standard Error 0.4 |
Secondary
Exercise-induced Oxidative Stress (i.e. Free Radical Concentrations and Levels of Lipid Peroxidation)
Free radicals and levels of lipid peroxidation were measured using electron paramagnetic spectroscopy and ELISA assays. A minimum value is 0 and a maximum value is 10. A higher value is a worse outcome.
Time frame: 8 weeks
| Arm | Measure | Value (MEAN) | Dispersion |
|---|---|---|---|
| Arm 1 | Exercise-induced Oxidative Stress (i.e. Free Radical Concentrations and Levels of Lipid Peroxidation) | 1.51 units on a scale | Standard Error 0.19 |
| Arm 2 | Exercise-induced Oxidative Stress (i.e. Free Radical Concentrations and Levels of Lipid Peroxidation) | 2.7 units on a scale | Standard Error 0.13 |
Secondary
Vascular Function Following the Antioxidant Intervention
The degree of vasodilation following the antioxidant intervention measured by Doppler ultrasound.
Time frame: 8 weeks
| Arm | Measure | Value (MEAN) | Dispersion |
|---|---|---|---|
| Arm 1 | Vascular Function Following the Antioxidant Intervention | 5.8 percentage of vasodilation | Standard Error 0.6 |
| Arm 2 | Vascular Function Following the Antioxidant Intervention | 8.2 percentage of vasodilation | Standard Error 0.6 |