Factors Involved in Obesity-related Inflammation and Insulin Resistance

Genetic and Nutritional Factors Involved in Obesity-related Inflammation and Insulin Resistance

Status

Terminated

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT01173705

Enrollment

Registered

2010-08-02

Start date

2011-06-30

Completion date

2014-01-31

Last updated

2017-01-20

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Inflammation, Insulin Resistance, Obesity

Keywords

Obesity, Insulin Resistance, Inflammation

Brief summary

In this project, we propose to recruit lean and obese subjects with different ethnic background (African Americans and Caucasians) to study the alterations of lipid and carbohydrate metabolism and determine whether these disturbances are linked to genetic, inflammatory, oxidative stress, and/or nutritional factors. Because systemic inflammation and insulin resistance are frequent features of obesity, we postulate that an unbalanced diet with high saturated- and low omega 3-fatty acids is linked to obesity-related inflammation and insulin resistance. We propose to investigate fatty acid metabolism and determine the links between fatty acid composition and oxidative stress in tissues of lean and obese subjects. We propose the following aims: Specific Aim 1: Evaluate nutrient intake in lean and obese subjects using the standard NHANES Food Questionnaire. Specific Aim 2: Evaluate the fatty acid composition, including omega-3, in adipose tissue depots, blood monocytes and skeletal muscle, and examine the relationship between omega-3 content and inflammatory and oxidative stress markers. Specific Aim 3: Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

18 Years to 65 Years

Healthy volunteers

Inclusion criteria

* 18-65 years of age * Undergoing elective abdominal or bariatric surgery

Exclusion criteria

* Intercurrent infections * Active cancer diagnosis within 5 years * Use of medications for diabetes or hyperlipidemia * Use of glucocorticoids or anti-inflammatory drugs

Design outcomes

Primary

Measure	Time frame	Description
Examine the relationship between omega-3 content and inflammatory and oxidative stress markers	5 years	We will evaluate fatty acid composition in adipose tissues depots, blood monocytes, and skeletal muscle to examine the relationship between omega-e content and inflammatory and oxidative stress markers.

Secondary

Measure	Time frame	Description
Evaluate nutrient intake	5 years	Using the NHANES Food Questionnaire, we will evaluate nutrient intakes of lean and obese individuals
Compare effects of omega-3 and saturated fatty acid supplementation on inflammatory and oxidative stress markers (in vitro)	5 years	Compare the effects of omega-3 and saturated FA supplementation on inflammatory and oxidative stress markers in vitro in adipose tissue explants, preadipocytes and monocyte culture.

Countries

United States

Outcome results