Rhinitis, Allergic, Perennial, Rhinitis, Allergic, Seasonal, Skin Prick Test
Conditions
Keywords
allergic rhinitis, concentration of diagnostic reagent, allergen extract, house dust mites (Dp), skin prick test, nasal allergen provocation test
Brief summary
The purpose of this study is to determine the appropriate concentration of diagnostic reagent of house dust mite (Dp) to be used in diagnostic allergy skin prick test among Thai population with allergic rhinitis.
Detailed description
The current allergy diagnostic reagent of house dust mites marketed in the USA is at 10,000 AU/ml. This is based on the IDEAL50 concept put forth by Turkeltaub et al, almost 3 decades ago. Such concentration has been shown to give large degree of false positives even in the US population. In Thailand, it was anecdotally observed that at such concentration, sizes of positive tests among atopic subjects were quite large and could lead to discomfort and a possible anaphylaxis in certain allergic individuals. Moreover, up to 40% of normal individuals could give positive reaction to this concentration. It is therefore desirable to reexamine the appropriate concentration of diagnostic reagent of house dust mites among the Thai population and will be the main objective of this research.
Interventions
None listed
Sponsors
Siriraj Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
15 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
allergic rhinitis * clinical diagnosis of allergic rhinitis * has skin prick test positive to standard Dp extract (wheal diameter 3 mm. or greater) * not take any medications that interfere the tests (skin prick test and nasal allergen provocation test) Inclusion Criteria:healthy subject * no underlying disease * not take any medications that interfere the tests (skin prick test and nasal allergen provocation test)
Exclusion criteria
* has skin prick test positive to standard Dp extract with wheal diameter more than 10 mm. * has chronic disease eg. HIV infection, neurological diseases, hypertension, cardiac diseases, pulmonary diseases, hepatobiliary diseases, renal diseases, endocrinological diseases, congenital malformation, malignancy * if has asthma, uncontrolled asthma symptom or PEF \< 70% * during pregnancy or lactation * has chronic skin disease * participate other project within 30 days * has abnormal anatomy of the nose
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| appropriate concentration of diagnostic reagent of house dust mite (Dp) | 15 minutes | to determine appropriate dilutional concentration of diagnostic reagent of house dust mite (Dp) that has high sensitivity and positive predictive value and wheal size not more than 10 mm. to be used in diagnosis allergic rhinitis in Thai population by skin prick test. |
Secondary
| Measure | Time frame |
|---|---|
| false positive rates of various dilutions of diagnostic reagent (house dust mite: Dp) that be used in skin prick test in Thai healthy population | 15 minutes |
| false negative rates of various dilutions of diagnostic reagent (house dust mite: Dp) that be used for diagnosis allergic rhinitis in Thai population by skin prick test | 15 minutes |
Countries
Thailand
Contacts
Primary ContactProfessor Pakit Vichyanond, MD
Backup ContactParawee Prottasan, MD
Outcome results
None listed