Healthy
Conditions
Keywords
Acute Exercise, Muscle Inflammation, CaHMB, HMB Free Acid Gel, Healthy Subjects
Brief summary
The proposed study will test efficacy of HMB in free acid gel to decrease muscle damage, diminish inflammatory response, and improve muscle strength recovery following an acute exercise stress. HMB in a free acid gel or in CaHMB capsule form will be administered as either a single dose before a bout of acute exercise or in multiple doses (one before and three daily doses for the 4 days following the acute exercise). We hypothesize that HMB in free acid gel will result in less muscle damage, diminished inflammatory response, and improved muscle strength recovery than either a placebo or CaHMB treatment following a bout of acute eccentric exercise. Our endpoints will include measurements of: (1) markers for muscle damage (CPK and LDH); (2) indicators of inflammation (CRP, TNF-α, IL-6, IL-1ra and IL-18); and (3) muscle strength recovery and soreness.
Interventions
DIETARY_SUPPLEMENTCaHMB
CaHMB capsules with each capsule containing 0.8 g of HMB
DIETARY_SUPPLEMENTHMB Free Acid Gel
HMB Free acid in a gel form with each dose supplying 0.8 g of HMB free acid
DIETARY_SUPPLEMENTPlacebo
Placebo capsules similar in size and color to the CaHMB capsules used in the study
DIETARY_SUPPLEMENTPlacebo Gel Dosage
A corn syrup based placebo with flavoring and taste similar to the HMB Free Acid Gel
Sponsors
Iowa State University
Metabolic Technologies Inc.
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Must live within 50 miles of Ames, Iowa * Free of cardiac, liver, pulmonary, and kidney disease * Free of chronic inflammatory disease including rheumatoid arthritis, osteoarthritis, inflammatory bowel disease, and psoriasis * Free diabetes mellitus * Not classified as morbidly obese (BMI must be less than 40) * Free of cardiac, liver, pulmonary, and kidney disease * No other serious medical illness * Normal menstrual cycle and not pregnant or lactating * Able to consume supplements and perform required exercise testing
Exclusion criteria
* Live more than 50 miles from Ames, Iowa * Presence of cardiac, liver, pulmonary, and kidney disease * Presence of chronic inflammatory disease including rheumatoid arthritis, osteoarthritis, inflammatory bowel disease, and psoriasis * Diabetes mellitus * Classified as morbidly obese (BMI is greater than 40) * No other serious medical illness * Abnormal menstrual cycle, pregnant or lactating * Not able to consume supplements and perform required exercise testing
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Decrease in muscle damage and inflammation | 4 days post eccentric exercise bout | Serum analysis for markers of muscle damage (CPK) and inflammation (IL-6, IL-18, IL1ra, TNF-a, CRP) will be measured at 24, 48, 72, and 96 h post exercise bout |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Improvement in strength recovery and decrease in soreness | 4 days post eccentric exercise bout | Muscle strength recovery and soreness will be measured at 24, 48, 72, and 96 h post exercise bout. |
Countries
United States
Outcome results
None listed